Capsaicinoid metabolism by the generalist Helicoverpa armigera and specialist H. assulta: Species and tissue differences,Pesticide Biochemistry and Physiology

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Capsaicinoid metabolism by the generalist Helicoverpa armigera and specialist H. assulta: Species and tissue differences
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-02-01 , DOI: 10.1016/j.pestbp.2019.11.013
Jiang Zhu ₁ , Kai Tian ₁ , Christopher A Reilly ₂ , Xinghui Qiu ₃

Affiliation

Helicoverpa armigera and H. assulta are two of the few insects that can feed on hot pepper fruits. Capsaicin and dihydrocapsaicin (i.e., capsaicinoids) are the principal pungent compounds in hot peppers. To explore possible molecular mechanisms of adaptation that allow these two species to consume capsaicinoids, the capacity of the three detoxification tissues (fat body, midgut, and Malpighian tubule) of the two pests, to metabolically degrade capsaicin and dihydrocapsaicin, was compared. The results showed that capsaicin and dihydrocapsaicin were metabolized by crude enzyme preparations from all three tissues of the two pests. Five metabolites of capsaicin, and five metabolites of dihydrocapsaicin were identified. Tissue and species differences in the degree of capsaicin and dihydrocapsaicin metabolism were observed. The specialist H. assulta had an overall greater capacity to degrade the capsaicinoids compared to the generalist H. armigera. Further, the midgut was the most significant contributor to capsaicinoid metabolism. The notably high specific activity in Malpighian tubules of H. armigera also further highlights the significance of this organ in xenobiotic detoxification. Alkyl hydroxylation and dehydrogenation were the main pathways for the oxidative biotransformation of both capsaicin and dihydrocapsaicin by cytochrome P450s. This study provides evidence that enhanced metabolic decomposition of capsaicinoids may be an adaptation explaining dietary preferences for Capsicum fruits by these two pests.

中文翻译：

全能棉铃虫和专家 H. assulta 的辣椒素代谢：物种和组织差异

Helicoverpa armigera 和 H. assulta 是少数可以吃辣椒果实的昆虫中的两种。辣椒素和二氢辣椒素（即类辣椒素）是辣椒中主要的刺激性化合物。为了探索使这两个物种消耗辣椒素的可能的适应分子机制，比较了两种害虫的三种解毒组织（脂肪体、中肠和马氏小管）代谢降解辣椒素和二氢辣椒素的能力。结果表明，辣椒素和二氢辣椒素均被两种害虫三种组织的粗酶制剂代谢。鉴定出辣椒素的五种代谢物和二氢辣椒素的五种代谢物。观察到辣椒素和二氢辣椒素代谢程度的组织和物种差异。专家H。与多面手 H. armigera 相比， assulta 具有更大的降解辣椒素的能力。此外，中肠是辣椒素代谢的最重要贡献者。棉铃虫 Malpighian 小管中显着的高比活性也进一步突出了该器官在异物解毒中的重要性。烷基羟基化和脱氢是细胞色素P450s氧化生物转化辣椒素和二氢辣椒素的主要途径。这项研究提供的证据表明，辣椒素类物质的代谢分解增强可能是解释这两种害虫对辣椒果实的饮食偏好的一种适应。中肠是辣椒素代谢的最重要贡献者。棉铃虫 Malpighian 小管中显着的高比活性也进一步突出了该器官在异物解毒中的重要性。烷基羟基化和脱氢是细胞色素P450s氧化生物转化辣椒素和二氢辣椒素的主要途径。这项研究提供的证据表明，辣椒素类物质的代谢分解增强可能是解释这两种害虫对辣椒果实的饮食偏好的一种适应。中肠是辣椒素代谢的最重要贡献者。棉铃虫 Malpighian 小管中显着的高比活性也进一步突出了该器官在异物解毒中的重要性。烷基羟基化和脱氢是细胞色素P450s氧化生物转化辣椒素和二氢辣椒素的主要途径。这项研究提供的证据表明，辣椒素类物质的代谢分解增强可能是解释这两种害虫对辣椒果实的饮食偏好的一种适应。烷基羟基化和脱氢是细胞色素P450s氧化生物转化辣椒素和二氢辣椒素的主要途径。这项研究提供的证据表明，辣椒素类物质的代谢分解增强可能是解释这两种害虫对辣椒果实的饮食偏好的一种适应。烷基羟基化和脱氢是细胞色素P450s氧化生物转化辣椒素和二氢辣椒素的主要途径。这项研究提供的证据表明，辣椒素类物质的代谢分解增强可能是解释这两种害虫对辣椒果实的饮食偏好的一种适应。

更新日期：2020-02-01

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