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Effects of ε-poly-l-lysine on vegetative growth, pathogenicity and gene expression of Alternaria alternata infecting Nicotiana tabacum
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-02-01 , DOI: 10.1016/j.pestbp.2019.11.005 He Liu 1 , Jianguang Chen 1 , Zihao Xia 1 , Mengnan An 1 , Yuanhua Wu 1
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-02-01 , DOI: 10.1016/j.pestbp.2019.11.005 He Liu 1 , Jianguang Chen 1 , Zihao Xia 1 , Mengnan An 1 , Yuanhua Wu 1
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Microbial secondary metabolites produced by Streptomyces are applied to control plant diseases. ε-poly-l-lysine (ε-PL) is a non-toxic food preservative, but the potential application of ε-PL as a microbial fungicide in agriculture has rarely been reported. In this study, Alternaria alternata (A. alternata) was used to reveal the effect and mode of action for ε-PL on the plant pathogenic fungi. The results showed that ε-PL effectively inhibited necrotic-lesion development caused by A. alternata on tobacco. Mycelial growth was also significantly inhibited in vitro by 100 μg/ml ε-PL using in vitro analysis. Moreover, 25 μg/ml ε-PL inhibited spore germination and induced abnormal morphological development of A. alternata hyphae. To clarify the molecular-genetic antifungal mechanisms, we selected several crucial genes involved in the development and pathogenesis of A. alternata and studied their expression regulated by ε-PL. Results of real-time quantitative PCR showed that a mycelium morphology and pathogenic process related cyclic adenosine monophosphate protein (cAMP) dependent protein kinase A (PKA), Alternaria alternata cAMP-dependent protein kinase catalytic subunit (AAPK1) and the early infection-related glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were down-regulated after ε-PL treatment. The results provide novel insights for the application of ε-PL in the control of plant diseases caused by A. alternata.
中文翻译：
ε-聚赖氨酸对侵染烟草的链格孢菌营养生长、致病力及基因表达的影响
链霉菌产生的微生物次生代谢产物用于防治植物病害。 ε-聚-L-赖氨酸（ε-PL）是一种无毒食品防腐剂，但ε-PL作为微生物杀菌剂在农业中的潜在应用鲜有报道。本研究以链格孢（A. alternata）为研究对象，揭示了ε-PL对植物病原真菌的作用及作用方式。结果表明，ε-PL能有效抑制烟草上链格孢菌引起的坏死病斑的发展。根据体外分析，100 μg/ml ε-PL 也能显着抑制菌丝生长。此外，25 μg/ml ε-PL 抑制孢子萌发并诱导A. alternata 菌丝形态发育异常。为了阐明抗真菌的分子遗传机制，我们选择了几个与A. alternata发育和发病机制有关的关键基因，并研究了它们受ε-PL调控的表达。实时定量PCR结果表明，菌丝形态和致病过程相关环磷酸腺苷蛋白（cAMP）依赖性蛋白激酶A（PKA）、链格孢cAMP依赖性蛋白激酶催化亚基（AAPK1）以及早期感染相关的甘油醛ε-PL 处理后，-3-磷酸脱氢酶 (GAPDH) 下调。研究结果为ε-PL在防治链格孢引起的植物病害中的应用提供了新的见解。
更新日期：2020-02-01
中文翻译：
ε-聚赖氨酸对侵染烟草的链格孢菌营养生长、致病力及基因表达的影响
链霉菌产生的微生物次生代谢产物用于防治植物病害。 ε-聚-L-赖氨酸（ε-PL）是一种无毒食品防腐剂，但ε-PL作为微生物杀菌剂在农业中的潜在应用鲜有报道。本研究以链格孢（A. alternata）为研究对象，揭示了ε-PL对植物病原真菌的作用及作用方式。结果表明，ε-PL能有效抑制烟草上链格孢菌引起的坏死病斑的发展。根据体外分析，100 μg/ml ε-PL 也能显着抑制菌丝生长。此外，25 μg/ml ε-PL 抑制孢子萌发并诱导A. alternata 菌丝形态发育异常。为了阐明抗真菌的分子遗传机制，我们选择了几个与A. alternata发育和发病机制有关的关键基因，并研究了它们受ε-PL调控的表达。实时定量PCR结果表明，菌丝形态和致病过程相关环磷酸腺苷蛋白（cAMP）依赖性蛋白激酶A（PKA）、链格孢cAMP依赖性蛋白激酶催化亚基（AAPK1）以及早期感染相关的甘油醛ε-PL 处理后，-3-磷酸脱氢酶 (GAPDH) 下调。研究结果为ε-PL在防治链格孢引起的植物病害中的应用提供了新的见解。
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