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A Pediococcus strain to rescue honeybees by decreasing Nosema ceranae- and pesticide-induced adverse effects
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-02-01 , DOI: 10.1016/j.pestbp.2019.11.006 Elodie Peghaire 1 , Anne Moné 1 , Frédéric Delbac 1 , Didier Debroas 1 , Frédérique Chaucheyras-Durand 2 , Hicham El Alaoui 1
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-02-01 , DOI: 10.1016/j.pestbp.2019.11.006 Elodie Peghaire 1 , Anne Moné 1 , Frédéric Delbac 1 , Didier Debroas 1 , Frédérique Chaucheyras-Durand 2 , Hicham El Alaoui 1
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Honeybees ensure a key ecosystemic service by pollinating many agricultural crops and wild plants. However, since few decades, managed bee colonies have declined worldwide. This phenomenon is considered to be multifactorial, with a strong emphasis on both parasites and pesticides. Infection by the parasite Nosema ceranae and exposure to pesticides can contribute to adverse effects, resulting in a perturbation of the honeybee physiology. We thus hypothesized that probiotic treatment could be promising to treat or prevent these disturbances. The aim of this study was to evaluate the effects of probiotics on N. ceranae-infected and intoxicated honeybees (by the insecticide thiamethoxam and the fungicide boscalid). For this purpose, experiments were conducted with five probiotics. Among them, Pediococcus acidilactici (PA) showed the best protective effect against the parasite and pesticides. PA significantly improved the infected honeybee lifespan as prophylactic and curative treatments (respectively 2.3 fold and 1.7 fold). Furthermore, the exposure to pesticides induced an increase of honeybee mortality compared with the control group (p < .001) that was restored by the PA treatment. Despite its beneficial effect on honeybee lifespan, the PA administration did not induce changes in the gut bacterial communities (neither in abundance or diversity). N. ceranae and the pesticides were shown to deregulate genes involved in honeybee development (vitellogenin), immunity (serine protease 40, defensin) and detoxification system (glutathione peroxidase-like 2, catalase), and these effects were corrected by the PA treatment. This study highlights the promising use of PA to protect honeybees from both pathogens and pesticides.
中文翻译：
一种片球菌菌株通过减少鼻子和杀虫剂引起的副作用来拯救蜜蜂
蜜蜂通过为许多农作物和野生植物授粉来确保关键的生态系统服务。然而，几十年来，管理的蜂群在全球范围内有所下降。这种现象被认为是多因素的，特别强调寄生虫和杀虫剂。寄生虫的感染和接触杀虫剂会导致不良反应，导致蜜蜂生理机能受到干扰。因此，我们假设益生菌治疗有望治疗或预防这些干扰。本研究的目的是评估益生菌对 N. ceranae 感染和中毒蜜蜂的影响（通过杀虫剂噻虫嗪和杀菌剂啶酰菌胺）。为此，用五种益生菌进行了实验。其中，乳酸片球菌（PA）对寄生虫和杀虫剂的保护效果最好。PA作为预防性和治疗性治疗显着提高了受感染蜜蜂的寿命（分别为2.3倍和1.7倍）。此外，与通过 PA 处理恢复的对照组 (p < .001) 相比，接触杀虫剂导致蜜蜂死亡率增加。尽管它对蜜蜂的寿命有益，但 PA 给药并未引起肠道细菌群落的变化（无论是丰度还是多样性）。N. ceranae 和杀虫剂被证明可以解除与蜜蜂发育（卵黄蛋白）、免疫（丝氨酸蛋白酶 40，防御素）和解毒系统（谷胱甘肽过氧化物酶样 2，过氧化氢酶）相关的基因的调节，并且这些影响通过 PA 处理得到纠正。
更新日期：2020-02-01
中文翻译：
一种片球菌菌株通过减少鼻子和杀虫剂引起的副作用来拯救蜜蜂
蜜蜂通过为许多农作物和野生植物授粉来确保关键的生态系统服务。然而，几十年来，管理的蜂群在全球范围内有所下降。这种现象被认为是多因素的，特别强调寄生虫和杀虫剂。寄生虫的感染和接触杀虫剂会导致不良反应，导致蜜蜂生理机能受到干扰。因此，我们假设益生菌治疗有望治疗或预防这些干扰。本研究的目的是评估益生菌对 N. ceranae 感染和中毒蜜蜂的影响（通过杀虫剂噻虫嗪和杀菌剂啶酰菌胺）。为此，用五种益生菌进行了实验。其中，乳酸片球菌（PA）对寄生虫和杀虫剂的保护效果最好。PA作为预防性和治疗性治疗显着提高了受感染蜜蜂的寿命（分别为2.3倍和1.7倍）。此外，与通过 PA 处理恢复的对照组 (p < .001) 相比，接触杀虫剂导致蜜蜂死亡率增加。尽管它对蜜蜂的寿命有益，但 PA 给药并未引起肠道细菌群落的变化（无论是丰度还是多样性）。N. ceranae 和杀虫剂被证明可以解除与蜜蜂发育（卵黄蛋白）、免疫（丝氨酸蛋白酶 40，防御素）和解毒系统（谷胱甘肽过氧化物酶样 2，过氧化氢酶）相关的基因的调节，并且这些影响通过 PA 处理得到纠正。
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