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Polyphenol-rich green tea extract induces thermogenesis in mice by a mechanism dependent on adiponectin signaling.
The Journal of Nutritional Biochemistry ( IF 4.8 ) Pub Date : 2019-12-11 , DOI: 10.1016/j.jnutbio.2019.108322
Anaysa Paola Bolin 1 , Celso Pereira Batista Sousa-Filho 2 , Marcelo Paradiso Marinovic 1 , Alice Cristina Rodrigues 1 , Rosemari Otton 3
Affiliation  

Adiponectin is downregulated in obesity negatively impacting the thermogenesis and impairing white fat browning. Despite the notable effects of green tea (GT) extract in the enhancement of thermogenesis, if its effects are being mediated by adiponectin has been scarcely explored. For this purpose, we investigated the role of adiponectin in the thermogenic actions of GT extract by using an adiponectin-knockout mice model. Male wild-type (WT) and knockout (AdipoKO) C57Bl/6 mice (3 months) were divided into 6 groups: mice fed a standard diet+gavage with water (SD WT, and SD AdipoKO), high-fat diet (HFD)+gavage with water (HFD WT, and HFD AdipoKO), and HFD + gavage with 500 mg/kg of body weight (BW) of GT extract (HFD + GT WT, and HFD + GT AdipoKO). After 20 weeks of experimentation, mice were euthanized and adipose tissue was properly removed. Our findings indicate that treatment with GT extract reversed complications of obesity in WT mice by decreasing final BW gain, adiposity index, adipocyte size and insulin resistance (IR). However, the action of the GT extract was not effective in reversing those markers in the AdipoKO mice, although GT acts independently in the reversal of IR. GT-treatment induced enhancement in energy expenditure (EE), BAT thermogenesis, and promoted browning phenotype in the subcutaneous WAT (scWAT) of WT mice. On the other hand, the thermogenic program was markedly impaired in BAT and scWAT of AdipoKO mice. Our outcomes unveiled adiponectin as a key direct signal for GT extract inducing adaptive thermogenesis and browning in scWAT.



中文翻译:

富含多酚的绿茶提取物通过依赖脂联素信号传导的机制诱导小鼠生热。

肥胖中脂联素被下调,对生热产生不利影响,并损害白色脂肪褐变。尽管绿茶(GT)提取物在生热增强中具有显着作用,但几乎没有研究过其作用是否由脂联素介导。为此,我们使用脂联素敲除小鼠模型研究了脂联素在GT提取物的生热作用中的作用。将雄性野生型(WT)和基因敲除(AdipoKO)C57Bl / 6小鼠(3个月)分为6组:用标准饮食+管饲水的小鼠(SD WT和SD AdipoKO),高脂饮食(HFD) )+用水(HFD WT和HFD AdipoKO)灌胃,以及HFD + 500毫克/千克体重(BW)的GT提取物(HFD + GT WT和HFD + GT AdipoKO)灌胃。实验20周后,对小鼠实施安乐死并适当去除脂肪组织。我们的发现表明,GT提取物治疗可通过降低最终BW增益,肥胖指数,脂肪细胞大小和胰岛素抵抗(IR)来逆转WT小鼠的肥胖并发症。然而,尽管GT在IR的逆转中独立起作用,但是GT提取物的作用在逆转AdipoKO小鼠中的那些标志物方面无效。GT处理可诱导WT小鼠皮下WAT(scWAT)的能量消耗(EE),BAT生热的增强,并促进褐变表型。另一方面,在AdipoKO小鼠的BAT和scWAT中,产热程序明显受损。我们的研究结果揭示了脂联素是GT提取物在scWAT中诱导适应性产热和褐变的关键直接信号。肥胖指数,脂肪细胞大小和胰岛素抵抗(IR)。然而,尽管GT在IR的逆转中独立起作用,但是GT提取物的作用在逆转AdipoKO小鼠中的那些标志物方面无效。GT处理诱导WT小鼠皮下WAT(scWAT)的能量消耗(EE),BAT生热的增强,并促进褐变表型。另一方面,AdipoKO小鼠的BAT和scWAT中的产热程序明显受损。我们的研究结果揭示了脂联素是GT提取物在scWAT中诱导适应性产热和褐变的关键直接信号。肥胖指数,脂肪细胞大小和胰岛素抵抗(IR)。然而,尽管GT在IR的逆转中独立起作用,但是GT提取物的作用在逆转AdipoKO小鼠中的那些标志物方面无效。GT处理可诱导WT小鼠皮下WAT(scWAT)的能量消耗(EE),BAT生热的增强,并促进褐变表型。另一方面,在AdipoKO小鼠的BAT和scWAT中,产热程序明显受损。我们的研究结果揭示了脂联素是GT提取物在scWAT中诱导适应性产热和褐变的关键直接信号。GT处理诱导WT小鼠皮下WAT(scWAT)的能量消耗(EE),BAT生热的增强,并促进褐变表型。另一方面,AdipoKO小鼠的BAT和scWAT中的产热程序明显受损。我们的研究结果揭示了脂联素是GT提取物在scWAT中诱导适应性产热和褐变的关键直接信号。GT处理可诱导WT小鼠皮下WAT(scWAT)的能量消耗(EE),BAT生热的增强,并促进褐变表型。另一方面,AdipoKO小鼠的BAT和scWAT中的产热程序明显受损。我们的研究结果揭示了脂联素是GT提取物在scWAT中诱导适应性产热和褐变的关键直接信号。

更新日期:2019-12-11
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