Early diagnosis of Hepatocellular Carcinoma (HCC) is an important means to raise the survival rate of patients. Multi-marker combined detection is a powerful tool of early HCC diagnosis. Traditional detection methods are not effective and accurate because it is difficult to achieve combined detection of multiple markers. In this paper, we selected Alpha Fetoprotein (AFP) and miRNA-125b as the combined detection markers to improve the simultaneously diagnostic sensitivity and specificity. The anti-AFP monoclonal antibody and the DNA probes paired with the miRNA-125b were modified on the surface of surface plasmon resonance (SPR) sensor respectively to specifically recognize AFP and miRNA-125b in serum. In order to enhance the SPR response signal and detection sensitivity, Double Antibody Sandwich Method (DASM) and S9.6 antibody enhanced method were applied to achieve low detection limit of the two markers. Experimental results showed that AFP (25-400 ng/mL) was accurately detected by DASM and the detection limit of miRNA-125b by S9.6 antibody enhanced method reached 123.044 pM. These results verified the feasibility of the multi-marker detection method in early diagnosis of HCC.

中文翻译：

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基于表面等离振子共振的早期肝细胞癌多标志物诊断方法。

肝细胞癌（HCC）的早期诊断是提高患者生存率的重要手段。多标记组合检测是早期HCC诊断的有力工具。传统的检测方法是无效且准确的，因为很难实现多个标记物的组合检测。在本文中，我们选择了甲胎蛋白（AFP）和miRNA-125b作为组合检测标记，以提高同时诊断的灵敏度和特异性。将抗AFP单克隆抗体和与miRNA-125b配对的DNA探针分别在表面等离子体共振（SPR）传感器的表面进行修饰，以特异性识别血清中的AFP和miRNA-125b。为了增强SPR响应信号和检测灵敏度，请使用双抗体夹心法（DASM）和S9。采用6种抗体增强方法，实现了两种标记物的低检测限。实验结果表明，DASM能够准确检测AFP（25-400 ng / mL），S9.6抗体增强方法检测到的miRNA-125b的检出限为123.044 pM。这些结果证实了多标记物检测方法在肝癌早期诊断中的可行性。