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All-in-one platform for salivary cotinine detection integrated with a microfluidic channel and an electrochemical biosensor.
Lab on a Chip ( IF 6.1 ) Pub Date : 2019-12-11 , DOI: 10.1039/c9lc01024f Kyungyeon Lee 1 , Taehee Yoon 1 , Hee-Seon Yang 2 , Sunyeong Cha 2 , Yong-Pil Cheon 2 , Leila Kashefi-Kheyrabadi 1 , Hyo-Il Jung 1
Lab on a Chip ( IF 6.1 ) Pub Date : 2019-12-11 , DOI: 10.1039/c9lc01024f Kyungyeon Lee 1 , Taehee Yoon 1 , Hee-Seon Yang 2 , Sunyeong Cha 2 , Yong-Pil Cheon 2 , Leila Kashefi-Kheyrabadi 1 , Hyo-Il Jung 1
Affiliation
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Medical disorders caused by second-hand smoke are a major public health concern worldwide. To estimate the level of second-hand smoke exposure, salivary diagnostics for cotinine analysis is a compelling alternative in conventional diagnostics using bio-fluids, such as blood and urine, owing to its simple and non-invasive collection method. However, there are several critical issues, such as tedious multisteps, demand for expertise, and field unavailability to collect and transport the purified saliva for further analysis. Here, an all-in-one platform is presented to simply collect real human saliva and directly deliver it onto the biosensing surface. The platform consists of a commercial cotton-swab-type collector, 3D-printed housing, and microfluidic channel integrated with an electrochemical competitive immunosensor to evaluate the level of salivary cotinine. The immunosensor is based on a competitive binding assay between cotinine-conjugated horseradish peroxidase (C-HRP) and cotinine for anti-cotinine binding sites. The current responses obtained from the HRP-thionine-H2O2 system decreased proportionally to the cotinine concentration. This immunosensor successfully detected its target over a range of 1 × 10-1 to 1 × 104 pg ml-1 with a low limit of detection of 6 × 10-2 pg ml-1 and a limit of quantification of 1 × 10-1 pg ml-1. In addition, the platform is applicable to various commercial cotton-swab-type saliva collectors and can successfully transfer the saliva in wide flow rates ranging from 0.1 to 30 ml min-1 without leakage or damage to the sensing surface. Furthermore, the practicality of the proposed platform was evaluated by measuring cotinine in real human saliva from eight non-smokers. The concentration of cotinine was from 45.7 to 890.8 pg ml-1, which was in good agreement with that measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The introduced all-in-one platform represented a reliable performance delivering simple and practical steps in salivary diagnostics.
中文翻译：
用于唾液可替宁检测的多合一平台，集成了微流通道和电化学生物传感器。
二手烟引起的医学疾病是全球主要的公共卫生问题。为了估计二手烟的暴露水平，唾液酸诊断可替宁分析是使用生物流体（例如血液和尿液）的常规诊断方法中的一种引人注目的替代方法，这是由于其简单且无创的收集方法。但是，存在几个关键问题，例如繁琐的多步操作，对专业知识的需求以及现场无法收集和运输纯化的唾液以进行进一步分析。在这里，提出了一个多合一的平台，可以简单地收集真实的人类唾液并将其直接传递到生物传感表面上。该平台包括一个商用棉签式收集器，3D打印的外壳，微流通道与电化学竞争性免疫传感器集成在一起，以评估唾液可替宁的水平。免疫传感器基于可替宁缀合的辣根过氧化物酶（C-HRP）和可替宁之间的竞争性结合测定，以测定抗子碱结合位点。从HRP-硫氨酸-H2O2系统获得的电流响应与可替宁浓度成比例地降低。该免疫传感器成功地在1×10-1至1×104 pg ml-1的范围内检测到其靶标，检测限低至6×10-2 pg ml-1，定量限为1×10-1 pg ml-1。此外，该平台适用于各种商用棉签型唾液收集器，并且可以成功地以0.1至30 ml min-1的宽流量传输唾液，而不会泄漏或损坏传感表面。此外，该平台的实用性是通过测量来自八个非吸烟者的真实人类唾液中的可替宁来评估的。可替宁的浓度为45.7至890.8 pg ml-1，与液相色谱-串联质谱法（LC-MS / MS）测得的浓度非常吻合。引入的多合一平台代表了可靠的性能，在唾液诊断中提供了简单实用的步骤。
更新日期：2020-02-13
中文翻译：
用于唾液可替宁检测的多合一平台，集成了微流通道和电化学生物传感器。
二手烟引起的医学疾病是全球主要的公共卫生问题。为了估计二手烟的暴露水平，唾液酸诊断可替宁分析是使用生物流体（例如血液和尿液）的常规诊断方法中的一种引人注目的替代方法，这是由于其简单且无创的收集方法。但是，存在几个关键问题，例如繁琐的多步操作，对专业知识的需求以及现场无法收集和运输纯化的唾液以进行进一步分析。在这里，提出了一个多合一的平台，可以简单地收集真实的人类唾液并将其直接传递到生物传感表面上。该平台包括一个商用棉签式收集器，3D打印的外壳，微流通道与电化学竞争性免疫传感器集成在一起，以评估唾液可替宁的水平。免疫传感器基于可替宁缀合的辣根过氧化物酶（C-HRP）和可替宁之间的竞争性结合测定，以测定抗子碱结合位点。从HRP-硫氨酸-H2O2系统获得的电流响应与可替宁浓度成比例地降低。该免疫传感器成功地在1×10-1至1×104 pg ml-1的范围内检测到其靶标，检测限低至6×10-2 pg ml-1，定量限为1×10-1 pg ml-1。此外，该平台适用于各种商用棉签型唾液收集器，并且可以成功地以0.1至30 ml min-1的宽流量传输唾液，而不会泄漏或损坏传感表面。此外，该平台的实用性是通过测量来自八个非吸烟者的真实人类唾液中的可替宁来评估的。可替宁的浓度为45.7至890.8 pg ml-1，与液相色谱-串联质谱法（LC-MS / MS）测得的浓度非常吻合。引入的多合一平台代表了可靠的性能，在唾液诊断中提供了简单实用的步骤。
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