BACKGROUND Infertility and gonadal dysfunction are well known side-effects by cancer treatment in males. In particularly, chemotherapy and radiotherapy induced testicular damage, resulting in prolonged azoospermia. However, information regarding therapeutics to treat spermatogenesis disturbance after cancer treatment is scarce. Recently, we demonstrated that Goshajinkigan, a traditional Japanese medicine, can completely rescue severe busulfan-induced aspermatogenesis in mice. In this study, we aimed to detect the effects of Goshajinkigan on aspermatogenesis after irradiation. METHODS This is animal research about the effects of traditional Japanese medicine on infertility after cancer treatment. C57BL/6 J male mice received total body irradiation (TBI: a single dose of 6Gy) at 4 weeks of age and after 60 days were reared a Goshajinkigan (TJ107)-containing or TJ107-free control diet from day 60 to day 120. Then, two untreated females were mated with a single male from each experimental group. On day 60, 120 and 150, respectively, the sets of testes and epididymis of the mice in each group after deep anesthetization were removed for histological and cytological examinations. RESULTS Histological and histopathological data showed that 6Gy TBI treatment decreased the fertility rate (4/10) in the control diet group; in contrast, in the TJ107-diet group, the fertility rate was 10/10 (p < 0.05 vs. 6Gy group). Supplementation with TJ107 was found to rescue the disrupted inter-Sertoli tight junctions via the normalization of claudin11, occludin, and ZO-1 expression and reduce serum anti-germ cell autoantibodies. CONCLUSIONS These findings show the therapeutic effect on TBI-induced aspermatogenesis and the recovering disrupted gonadal functions by supplementation with TJ107.

中文翻译：

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传统日本药Goshajinkigan在辐射诱导的小鼠生精中的功效。

背景技术不育和性腺功能障碍是男性通过癌症治疗的众所周知的副作用。特别地，化学疗法和放射疗法引起睾丸损伤，导致无精症时间延长。然而，关于治疗癌症后精子发生障碍的疗法的信息很少。最近，我们证明了Goshajinkigan，一种传统的日本药物，可以完全拯救严重的白消安诱导的小鼠生精。在这项研究中，我们旨在检测Goshajinkigan对辐射后的生精作用。方法这是一项有关传统日本药物对癌症治疗后不孕症影响的动物研究。C57BL / 6 J雄性小鼠接受全身照射（TBI：在第60天至第120天饲养4周龄和60天后的单剂量6Gy），饲养含Goshajinkigan（TJ107）或不含TJ107的对照饮食。然后，将两只未经治疗的雌性与每只雌性交配实验组。分别在第60、120和150天，深麻醉后各组小鼠的睾丸和附睾组被取出进行组织学和细胞学检查。结果组织学和组织病理学数据显示，对照饮食组6Gy TBI治疗降低了生育率（4/10）；相反，在TJ107饮食组中，生育率是10/10（p <0.05 vs. 6Gy组）。发现通过补充claudin11，occludin，和ZO-1表达并降低血清抗生殖细胞自身抗体。结论这些发现表明通过补充TJ107对TBI诱导的生精作用和恢复性腺功能的治疗作用。