BACKGROUND Grey mould is an important plant disease worldwide, caused by Botrytis cinerea, resulting in serious economic loss. Wuyiencin, a low toxicity, high efficiency, and broad-spectrum agricultural antibiotic, has been demonstrated effectiveness against B. cinerea. RESULTS Wuyiencin treatment inhibited growth and sporulation of B. cinerea, specifically altering hypha morphology and intracellular structures. These changes were accompanied by differential expression (fold change > 2.0) of 316 proteins identified by iTRAQ-labelling LC-MS/MS analysis (P < 0.05). Up-regulation of 14 proteins, including carbohydrate metabolism proteins and cell wall stabilization proteins, was validated by parallel reaction monitoring (PRM). Down-regulation of 13 proteins was validated by PRM, including regulators of energy metabolism, nucleotide/protein synthesis, and the biosynthesis of mediators of plant stress and decay. CONCLUSION Our results confirm the inhibitory biological effects of wuyiencin on B. cinereal and elaborate on the differentially expressed proteins and associated pathways implicated in the capacity of wuyiencin to debilitate the growth and pathogenicity of grey mould. This study provides validated candidates for further targeted exploration with the goal of optimizing wuyiencin as a safe, low-toxicity agent for biological control.

中文翻译：

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基于iTRAQ的蛋白质组学分析揭示了由Wuyiencin控制的灰葡萄孢菌的机制。

背景技术灰霉病是世界范围内由灰葡萄孢（Botrytis cinerea）引起的重要植物病，导致严重的经济损失。Wuyiencin是一种低毒，高效且广谱的农业抗生素，已被证明对灰葡萄孢菌有效。结果Wuyiencin治疗抑制灰葡萄孢菌的生长和孢子形成，特别是改变菌丝形态和细胞内结构。这些变化伴随着iTRAQ标记LC-MS / MS分析鉴定出的316种蛋白质的差异表达（倍数变化> 2.0）（P <0.05）。通过平行反应监测（PRM）验证了14种蛋白的上调，包括碳水化合物代谢蛋白和细胞壁稳定蛋白。PRM验证了13种蛋白质的下调，包括能量代谢的调节剂，核苷酸/蛋白质合成，以及植物胁迫和衰变介体的生物合成。结论我们的研究结果证实了Wuyiencin对B. cinereal的抑制生物学作用，并详细阐明了Wuyiencin削弱灰霉菌生长和致病性的能力所涉及的差异表达蛋白和相关途径。这项研究为进一步优化乌霉素的安全性，低毒性的生物防治剂提供了经过验证的候选物，可用于进一步的靶向探索。并详细说明差异表达的蛋白质和相关途径，这与五邑霉素削弱灰霉菌的生长和致病性有关。这项研究为进一步优化乌叶霉素作为一种安全，低毒性的生物防治剂提供了经过验证的候选对象，可用于进一步的有针对性的探索。并详细说明差异表达的蛋白质和相关途径，这与乌霉素的作用削弱了灰霉菌的生长和致病性有关。这项研究为进一步优化乌叶霉素作为一种安全，低毒性的生物防治剂提供了经过验证的候选对象，可用于进一步的有针对性的探索。