A ruthenium polypyridine complex, [Ru(mptpy)(Mmptpy)Cl][PF6]2 (Ru1, mptpy = 4-(4-methylphenyl)-2,2′,6′,2″-mptpyridine, Mmptpy = 2-[4-(4-methylphenyl)-2,2′-bipyridin-6-yl]-1-methylpyridinum), was synthesised and characterised. The structure of the complex was determined by single-crystal X-ray crystallography. The ruthenium(II) core has an octahedral coordination environment. The interactions of the complex with DNA were analysed by UV–Vis and fluorescence spectroscopy, revealing a high binding affinity for double-stranded DNA. DNA photocleavage studies monitored by agarose gel electrophoresis showed that the complex cleaved DNA efficiently, whilst mechanistic studies showed that the complex produced reactive oxygen species including singlet oxygen, superoxide anions, and carbon-based radicals that cleave DNA. The complex also showed notable antiproliferation activity towards cancer cells in methyl thiazolyl tetrazolium assays.

中文翻译：

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带有甲基化多吡啶配体的钌 (II) 复合物：合成、DNA 结合和抗增殖活性

钌多吡啶络合物，[Ru(mptpy)(Mmptpy)Cl][PF6]2 (Ru1, mptpy = 4-(4-methylphenyl)-2,2',6',2″-mptpyridine, Mmptpy = 2-[合成并表征了 4-(4-甲基苯基)-2,2'-联吡啶-6-基]-1-甲基吡啶。配合物的结构由单晶 X 射线晶体学确定。钌（II）核具有八面体配位环境。复合物与 DNA 的相互作用通过 UV-Vis 和荧光光谱分析，揭示了对双链 DNA 的高结合亲和力。通过琼脂糖凝胶电泳监测的 DNA 光裂解研究表明，该复合物有效地裂解了 DNA，而机理研究表明，该复合物产生了活性氧，包括单线态氧、超氧阴离子和可裂解 DNA 的碳基自由基。