Phosphoinositide-specific phospholipase C signalling mediates expression of two phenylalanine ammonia lyase genes induced by salicylic acid in Capsicum chinense cells,Journal of Plant Biochemistry and Biotechnology

当前位置： X-MOL 学术 › J. Plant Biochem. Biot. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Phosphoinositide-specific phospholipase C signalling mediates expression of two phenylalanine ammonia lyase genes induced by salicylic acid in Capsicum chinense cells
Journal of Plant Biochemistry and Biotechnology ( IF 1.6 ) Pub Date : 2019-11-19 , DOI: 10.1007/s13562-019-00544-w
Beatriz A. Rodas-Junco , Víctor González-Mendoza , Armando Muñoz-Sánchez , Wilbert Poot-Poot , Felipe Vázquez-Flota , S. M. Teresa Hernández-Sotomayor

The role of salicylic acid (SA) in the plant stress response is mediated by phenylalanine ammonia lyase (PAL), a key enzyme in the phenylpropanoid pathway in Capsicum chinense cells. However, the relationship between PAL expression in response to SA and the precise involvement of the phosphoinositide-phospholipase C (PI-PLC) transduction pathway linking these effects remains unclear. A pharmacological approach was used to investigate the role of the PI-PLC pathway in the transcriptional regulation of two different PAL gene members in C. chinense suspension cells exposed to SA. Two putative C. chinense PAL genes (CchPAL1 and CchPAL5) were identified after clustering as an independent group in a phylogenetic tree built with 67 Solanaceae genes, corresponding to six species. The expression of CchPAL1 and CchPAL5 showed differential patterns in C. chinense cells exposed to salicylic acid. Moreover, PLC inhibitors, such as neomycin and U73122, differentially modified the expression profiles. These findings suggest that the PI-PLC pathway participates in the activation of preferred PAL genes in response to SA in Capsicum cells.

更新日期：2019-11-19

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11