The UPLC-Q-TOF–MS/MS analysis of ethanol extract of leaf confirmed the presence of important flavonoids like rutin (RT 5.95), hesperidin (RT 5.99), quercitrin (RT 6.33) and kaempferol-3-O-glucoside (RT 6.82). Photo-induced green synthesis of silver nanoparticles was reported in Nycanthes arbor-tristis. Nanoparticles were efficiently synthesized after mixing of 1% ethanol leaf extract and 1 mM silver nitrate solution under 10 min light exposure at room temperature. The UV–Vis spectrum of the brown colored reaction mixture showed sharp surface plasmon resonance at 460 nm. It confirmed the biosynthesis of silver nanoparticles. Nanoparticles were characterized by using SEM, TEM, and EDX. The results suggest that synthesized nanoparticles lie in the range of 10–50 nm. XRD, SAED and AFM data confirmed the polycrystalline nature of biosynthesized nanoparticles. Silver nanoparticles exhibited strong antibacterial activity than extract. All bacterial clinical isolates were sensitive to nanoparticles. Methicilin resistant Staphylococcus aureus was most susceptible than other isolates. The silver nanoparticles also exhibited good free radical scavenging activity (EC₅₀ 670.67 ± 15.70 µg/ml).

中文翻译：

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从夜蛾叶提取物合成绿色纳米银及其抗氧化性，抗菌反应的评价。

叶片乙醇提取物的UPLC-Q-TOF-MS / MS分析证实了重要的类黄酮的存在，如芦丁（RT 5.95），橙皮苷（RT 5.99），槲皮苷（RT 6.33）和山奈酚3-O-葡萄糖苷（RT 6.82）。光致银纳米粒子的绿色合成报道在Nycanthes乔木- tristis。在室温下暴露10分钟后，将1％乙醇叶提取物和1 mM硝酸银溶液混合后，可以有效地合成纳米粒子。棕色反应混合物的UV-Vis光谱在460 nm处显示出清晰的表面等离子体共振。它证实了银纳米颗粒的生物合成。通过使用SEM，TEM和EDX表征纳米颗粒。结果表明，合成的纳米颗粒在10–50 nm范围内。XRD，SAED和AFM数据证实了生物合成纳米颗粒的多晶性质。银纳米颗粒显示出比提取物更强的抗菌活性。所有细菌临床分离株均对纳米颗粒敏感。耐甲氧西林的金黄色葡萄球菌比其他分离株最易感。银纳米颗粒还表现出良好的自由基清除活性（EC ₅₀ 670.67±15.70 µg / ml）。