Chitosan oligosaccharide (COS), a water-soluble carbohydrate obtained from chemical or enzymatic hydrolysis of chitosan, has similar structure and properties to non-toxic, biocompatible, and biodegradable chitosan. However, COS has many advantages over chitosan due to its low molecular weight and high water solubility. In the current work, COS was incorporated in the laccase-catalyzed polymerization of hydroquinone. The laccase-catalyzed polymerization of hydroquinone with or without COS was investigated by using simple structure of glucosamine hydrochloride as an alternative to COS to understand the mechanism of COS-incorporated polymerization of hydroquinone. Although polyhydroquinone can be regarded as the polymeric colorant with dark brown color, there is no affinity or chemical bonding between polyhydroquinone and cotton fibers. Cotton fabrics were successfully in-situ dyed into brown color through the laccase-catalyzed polymerization of hydroquinone by incorporating with COS as a template. The presence of COS enhanced the dye uptake of polyhydroquinone on cotton fibers due to high affinity of COS to cotton and covalent bonding between COS and polyhydroquinone during laccase catalysis. This novel approach not only provides a simple route for the biological coloration of cotton fabrics but also presents a significant way to prepare functional textiles with antibacterial property.

壳聚糖低聚糖（COS）是一种从壳聚糖的化学或酶促水解过程中获得的水溶性碳水化合物，其结构和特性与无毒，生物相容性和可生物降解的壳聚糖相似。但是，由于其分子量低和水溶性高，因此与壳聚糖相比具有许多优势。在当前的工作中，COS被并入漆酶催化的氢醌聚合中。通过使用葡萄糖胺盐酸盐的简单结构作为COS的替代物，研究了漆酶催化的带或不带COS的对苯二酚聚合反应，以了解结合COS的氢醌聚合机理。尽管聚对苯二酚可以被视为具有深褐色的聚合物着色剂，但聚对苯二酚和棉纤维之间没有亲和力或化学键合。以COS为模板，通过漆酶催化的对苯二酚聚合反应成功地将棉织物原位染色为棕色。COS的存在增强了棉纤维上聚氢醌对染料的吸收，这是因为COS对棉花的亲和力很高，并且在漆酶催化过程中COS和聚氢醌之间存在共价键合。这种新颖的方法不仅为棉织物的生物染色提供了简单的途径，而且为制备具有抗菌性能的功能性纺织品提供了重要的途径。COS的存在增强了棉纤维上聚氢醌对染料的吸收，这是因为COS对棉花的亲和力很高，并且在漆酶催化过程中COS和聚氢醌之间存在共价键合。这种新颖的方法不仅为棉织物的生物染色提供了简单的途径，而且为制备具有抗菌性能的功能性纺织品提供了重要的途径。COS的存在增强了棉纤维上聚氢醌对染料的吸收，这是因为COS对棉花的亲和力很高，并且在漆酶催化过程中COS和聚氢醌之间存在共价键合。这种新颖的方法不仅为棉织物的生物染色提供了简单的途径，而且为制备具有抗菌性能的功能性纺织品提供了重要的途径。