Nostoc microscopicum an effective extracellular polymers (EPs) synthesizer among cyanobacteria was isolated, and its elementary morphological features were defined with the aid of light microscope and CLSM. Bioseparation of EPs from 44 days-aged culture gave 0.90 g/L of the dry powdered extract. Chemical quantification of EPs showed the presence of 550 mg/g of carbohydrate and 395 mg/g of protein. HPLC results depicted the presence of mannose (monomer sugar), fucose (hexose deoxy sugar), mannitol (sugar alcohol) and N- acetylglucosamine (glycoprotein) in the EPs. The vibration-based spectrum produced by FT-IR proves the β-Sheet structure of EPs glycoprotein and the size as 45 kDa by performing SDS-PAGE. Bactericidal activity evaluation of EPs on Pseudomonas aeruginosa and Staphylococcus aureus co-expressed the MIC value as 125 μg/mL, while zone of inhibition was 12 mm for Gram-negative and 8 mm for Gram-positive bacteria. Biofilm inhibition assay was effective in 1.0 mg/mL concentration of EPs in both bacterial strains with a mean rate of 60 percentages which was further confirmed using confocal laser scanning microscopic imaging. This natural polymeric extract of Nostoc microscopicum indicates its possible applications in bactericidal and biofilm inhibition.

中文翻译：

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糖蛋白提示来自Nostoc microscopicum的细胞外聚合物的合理杀菌和抗生物膜产物。

发菜microscopicum蓝藻中一个有效的胞外聚合物（EPS）合成，分离，及它的基本形态特征用光学显微镜和CLSM的帮助限定。从44天龄的培养物中对EP进行生物分离，得到0.90 g / L的干粉状提取物。EP的化学定量显示存在550 mg / g的碳水化合物和395 mg / g的蛋白质。HPLC结果描述了EP中存在甘露糖（单体糖），岩藻糖（己糖脱氧糖），甘露醇（糖醇）和N-乙酰氨基葡糖（糖蛋白）。FT-IR产生的基于振动的光谱通过执行SDS-PAGE证明了EPs糖蛋白的β-Sheet结构和大小为45 kDa。EPs对铜绿假单胞菌和铜绿假单胞菌的杀菌活性评价。金黄色葡萄球菌共表达的MIC值为125μg/ mL，而革兰氏阴性菌的抑制区为12 mm，革兰氏阳性菌的抑制区为8 mm。生物膜抑制试验在两种细菌菌株中以1.0 mg / mL的EP浓度均有效，平均比率为60％，这已通过共聚焦激光扫描显微成像得到了进一步证实。这种天然的Nostoc microscopicum的聚合物提取物表明其在杀菌和生物膜抑制中的可能应用。