This study aims to investigate the protective effects of silymarin (Sm) in thioacetamide (TAA)-related liver damage. What makes this study special is that it attempts to determine the expression of changes in the liver at the level of gene expression. Routine liver damage markers were compared with changes in the levels of microRNA (miRNA) known as new biomarkers. With this in mind, we divided the rats into four groups including control, TAA, Sm + TAA (50 + 50 mg/kg), and Sm + TAA (100 + 50 mg/kg). Blood and tissue samples belonging to the rats were collected in consideration of morphological, immunohistochemistry, miRNAs levels, and biochemical evaluations. Our study results showed that miR-122, miR-192, and miR-194 levels had decreased in the experimental groups given TAA, whereas miR-122, miR-192, and miR-194 levels had increased in the doses of Sm + TAA-given group. Therefore, Sm treatment undertaken before exposure to the toxin successfully altered its effects upon the study animals.

中文翻译：

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水飞蓟素对硫代乙酰胺诱导的肝损伤的保护作用：miR-122，miR-192和miR-194水平的测量。

这项研究旨在调查水飞蓟素（Sm）对硫代乙酰胺（TAA）相关的肝损伤的保护作用。这项研究与众不同的是，它试图确定基因表达水平上肝脏变化的表达。将常规肝损伤标志物与称为新生物标志物的微小RNA（miRNA）水平的变化进行比较。考虑到这一点，我们将大鼠分为四组，包括对照组，TAA，Sm + TAA（50 + 50 mg / kg）和Sm + TAA（100 + 50 mg / kg）。考虑到形态，免疫组织化学，miRNA水平和生化评估，收集了属于大鼠的血液和组织样本。我们的研究结果表明，在给予TAA的实验组中，miR-122，miR-192和miR-194的水平有所降低，而miR-122，miR-192，Sm + TAA给予的剂量组中miR-194水平升高。因此，在暴露于毒素之前进行的Sm处理成功地改变了其对研究动物的影响。