An established ferric reducing antioxidant power (FRAP) assay was optimised by preparation of the derivatisation reagent in 300 mM formate instead of 300 mM acetate conditions, resulting in increased sensitivity signal to noise responses by up to five to ten times. The quantitative protocol for selective detection of antioxidants via a HPLC post column derivatisation (PCD) technique using the 300 mM formate FRAP reagent conditions was then transformed into a high-speed qualitative screening protocol by utilizing an emerging technology ‘reaction flow (RF) chromatography’. Reaction flow chromatography’s ability to screen for total antioxidant capacity with additional peak specificity/profile information of active peaks could be achieved in under 2 min.

中文翻译：

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通过反应流色谱和三氧化二铁还原抗氧化能力分析确定总抗氧化剂容量的峰特异性

通过在300 mM甲酸盐（而不是300 mM乙酸盐）中制备衍生试剂来优化已建立的三价铁还原抗氧化剂能力（FRAP）分析，从而使信号对噪声响应的灵敏度提高了五至十倍。通过使用300 mM甲酸盐FRAP试剂条件的HPLC柱后衍生化（PCD）技术选择性检测抗氧化剂的定量方案，然后通过利用新兴技术“反应流（RF）色谱”转化为高速定性筛选方案。在不到2分钟的时间内即可实现反应流色谱法筛选总抗氧化剂能力以及活性峰的额外峰特异性/特征信息的能力。