Many medical-related scientific discoveries arise from trial-error patterns where the processes involved must be refined and modified continuously before any product could be able to reach the final costumers. One of the elements affecting negatively these processes is the inaccuracy of two-dimension (2D) standard culture systems, carried over in plastic plates or similar, in replicating complex environments and patterns. Consequently, animal tests are required to validate every in vitro finding, at the expenses of more funds and ethical issues. A possible solution relies in the implementation of three-dimension (3D) culture systems as a fitting gear between the 2D tests and in vivo tests, aiming to reduce the negative in vivo outcomes. These 3D structures are depending from the comprehension of the extracellular matrix (ECM) and the ability to replicate it in vitro. In this article a comparison of efficacies between these two culture systems was taken as subject, human mesenchymal stem cells (hMSCs) was utilized and a hybrid scaffold made by a blend of chitosan, gelatin and biomineralized gelatin was used for the 3D culture system.

中文翻译：

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量身定制的3D混合支架和2D标准培养物之间的人间充质干细胞成骨诱导的差异。

许多与医学有关的科学发现源于试验错误模式，在此模式下，必须不断完善和修改所涉及的过程，然后才能将任何产品送达最终客户。对这些过程产生负面影响的因素之一是二维（2D）标准培养系统的不准确性，该系统在塑料板或类似板上携带，无法复制复杂的环境和图案。因此，需要进行动物试验以验证每个体外发现，但要花费更多的资金和道德问题。一种可能的解决方案依赖于将三维（3D）培养系统作为2D测试和体内测试之间的合适装置，以减少负面的体内结果。这些3D结构取决于细胞外基质（ECM）的理解以及在体外复制它的能力。在本文中，将这两种培养系统之间的效率进行比较，以人间充质干细胞（hMSCs）为基础，将壳聚糖，明胶和生物矿化明胶的混合物制成的混合支架用于3D培养系统。