Abstract

Mesenchymal stem cells (MSCs) has been used as one of the new cell sources in osteochondral tissue engineering. It has been well known that control of their differentiation into chondrocytes plays a key role in developing engineered cartilages. Therefore, this study aims to develop a fundamental protocol to control the differentiation and proliferation of MSCs to construct an engineered cartilage. We compared the effects of three different culture conditions on cell proliferation, extracellular matrix formation and the mechanical response of engineered cartilage constructed using a collagen-based hybrid scaffold and human MSCs. The experimental results clearly showed that the combined culture condition of the chondrogenic differentiation culture and the chondrocyte growth culture exhibited statistically significant cell proliferation, ECM formation and stiffness responses as compared to the other two combinations. It is thus concluded that the combination of the differentiation culture with the subsequent growth culture is recommended as the culture condition for chondrogenic tissue engineering using hMSCs.

中文翻译：

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培养条件对胶原混合支架和人间充质干细胞构建的工程软骨力学和生物学特性的影响

抽象的

间充质干细胞（MSCs）已被用作骨软骨组织工程中的新细胞来源之一。众所周知，控制其向软骨细胞的分化在发展工程软骨中起关键作用。因此，本研究旨在开发一种基本协议来控制MSC的分化和增殖，以构建工程软骨。我们比较了三种不同培养条件对细胞增殖，细胞外基质形成以及使用基于胶原的混合支架和人MSC构建的工程软骨的机械反应的影响。实验结果清楚地表明，软骨分化培养和软骨细胞生长培养的组合培养条件具有统计学意义的细胞增殖，与其他两种组合相比，ECM的形成和刚度响应。因此得出的结论是，推荐将分化培养与随后的生长培养相结合作为使用hMSC进行软骨组织工程化的培养条件。