BACKGROUND Graft-versus-host disease (GVHD) is one of the most complex complications after allogeneic stem cell transplantation. Current standard of grading system is based on clinical symptoms in skin, liver and intestinal. However, it's difficult to differ GVHD and its extent just by clinical manifestation. Here we retrospectively analyzed cell immune function in patients implemented allogeneic stem cell transplantation in Ningbo first Hospital from Jan 2013 to Jan 2018. RESULTS the data are collected from 51 patients (mean age was 42; 45.1% women). The average NK cell percentage was 39.31% in severe GVHD (Grade III-IV), was 16.98% in mild GVHD (GradeI-II), while was 21.15% in No GVHD group. The statistical analysis showed difference among each grade. Further analysis was performed in Antithymocyte globulin (ATG) treated group and control group. We showed NK Cell percentage was sharply different in ATG treated group: 47.34% in severe GVHD, 11.98% in mild GVHD group, while 18.3% in no GVHD group. However, in control group, the average percentage of NK cells was 23.27% in severe GVHD, was 23.22%in mild GVHD group, while was 21.13% in no GVHD group. CONCLUSION The data supports that ATG can prevent GVHD by increasing NK cell percentage. The percentage of NK cell seemed to be a useful probe to evaluate the severity of GVHD in allogeneic stem cell transplantation patients using ATG in pretreatment.

中文翻译：

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在预处理方案中，使用抗胸腺细胞球蛋白（ATG）进行同种异体干细胞移植后，NK细胞可预测患者急性移植物抗宿主病的严重性。

背景技术移植物抗宿主病（GVHD）是同种异体干细胞移植后最复杂的并发症之一。当前的分级系统标准基于皮肤，肝脏和肠道的临床症状。但是，仅凭临床表现很难区分GVHD及其程度。在这里，我们回顾性分析了2013年1月至2018年1月在宁波市第一医院实施异体干细胞移植的患者的细胞免疫功能。结果数据来自51例患者（平均年龄42岁；女性45.1％）。严重GVHD（III-IV级）的平均NK细胞百分比为39.31％，轻度GVHD（I-II级）的平均NK细胞百分比为16.98％，而无GVHD组的平均NK细胞百分比为21.15％。统计分析表明每个年级之间存在差异。在抗胸腺细胞球蛋白（ATG）治疗组和对照组中进行了进一步的分析。我们显示，ATG治疗组的NK细胞百分比显着不同：严重GVHD组为47.34％，轻度GVHD组为11.98％，而无GVHD组为18.3％。然而，在对照组中，重度GVHD中NK细胞的平均百分比为23.27％，轻度GVHD组中为23.22％，而无GVHD组中为21.13％。结论数据支持ATG可以通过增加NK细胞百分比来预防GVHD。NK细胞的百分比似乎是评估使用ATG进行预处理的同种异体干细胞移植患者中GVHD严重程度的有用探针。重度GVHD中NK细胞的平均百分比为23.27％，轻度GVHD组中为23.22％，无GVHD组中为21.13％。结论数据支持ATG可以通过增加NK细胞百分比来预防GVHD。NK细胞的百分比似乎是评估使用ATG进行预处理的同种异体干细胞移植患者中GVHD严重程度的有用探针。重度GVHD中NK细胞的平均百分比为23.27％，轻度GVHD组中为23.22％，无GVHD组中为21.13％。结论数据支持ATG可以通过增加NK细胞百分比来预防GVHD。NK细胞的百分比似乎是评估使用ATG进行预处理的同种异体干细胞移植患者中GVHD严重程度的有用探针。