BACKGROUND Radix Astragali and Radix Angelicae Sinensis are two herbs that compose Danggui Buxue Tang (an herbal formula for treatment of anemia diseases). In this study, we explored the molecular mechanism and effective targets to immune destruction of bone marrow (BM) cells treated with Radix Astragali, Radix Angelicae Sinensis or a combination of two agents. The potential synergic advantages of two herbs should also be explored. METHODS The constituents of Radix Astragali and Radix Angelicae Sinensis were analyzed by high performance liquid chromatography-electrospray ionization/mass spectrometer system BM cells were separated from limbs of BALB/c mice, and immune destruction was induced with IFN-γ. The percentages of hematopoietic stem cells (HSCs) and CD3+ T cells were detected by flow cytometry. The distribution of T-bet and changes in the combination of SAP and SLAM in BM cells were observed by immunofluorescence. Western blotting was used to assay the expression of key molecules of the eIF2 signaling pathway in BM cells. RESULTS Seven constituents of Radix Astragali and six constituents of Radix Angelicae Sinensis were identified. The percentages of HSCs increased significantly after treatment with Radix Angelicae Sinensis, especially at high concentrations. The percentages of CD3+ T cells were significantly decreased after Radix Astragali and Radix Angelicae Sinensis treatment. However, the synergistic function of two-herb combinations was superior to that of the individual herbs alone. The distribution of T-bet in BM cells was decreased significantly after Radix Angelicae Sinensis treatment. The number of SLAM/SAP double-stained cells was increased significantly after Radix Astragali treatment at low concentrations. The phosphorylation levels of eIF2α were also reduced after Radix Astragali and Radix Angelicae Sinensis treatment. CONCLUSIONS Radix Astragali and Radix Angelicae Sinensis could intervene in the immunologic balance of T lymphocytes, inhibit the apoptosis of BM cells induced by immune attack, restore the balance of the T cell immune response network and recover the hematopoietic function of HSCs. The synergistic effects of Radix Astragali and Radix Angelicae Sinensis were superior to those of each herb alone.

中文翻译：

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黄芪和当归的组合减弱了IFN-γ诱导的骨髓细胞造血功能的免疫破坏。

背景技术黄芪和当归是组成当归补血汤（一种治疗贫血疾病的草药配方）的两种草药。在这项研究中，我们探讨了黄芪，当归或两种药物联合处理对骨髓（BM）细胞免疫破坏的分子机制和有效靶标。还应该探索两种草药的潜在协同优势。方法采用高效液相色谱-电喷雾电离/质谱联用系统分析黄芪和当归的成分，从BALB / c小鼠四肢分离BM细胞，用IFN-γ诱导免疫破坏。通过流式细胞术检测造血干细胞（HSCs）和CD3 + T细胞的百分比。通过免疫荧光观察了BM细胞中T-bet的分布以及SAP和SLAM组合的变化。Western印迹法用于检测BM细胞中eIF2信号通路关键分子的表达。结果鉴定出黄芪7个成分和当归6个成分。当归当归处理后，HSC的百分比显着增加，尤其是在高浓度下。黄芪和当归处理后CD3 + T细胞百分比明显降低。但是，两种草药组合的协同作用优于单独的草药。当归处理后，T-bet在BM细胞中的分布明显降低。低剂量黄芪处理后SLAM / SAP双染色细胞数量明显增加。黄芪和当归处理后，eIF2α的磷酸化水平也降低。结论黄芪和当归可以干预T淋巴细胞的免疫平衡，抑制免疫攻击诱导的BM细胞凋亡，恢复T细胞免疫反应网络的平衡，恢复HSCs的造血功能。黄芪和当归的协同作用优于单独的每种药草。结论黄芪和当归可以干预T淋巴细胞的免疫平衡，抑制免疫攻击诱导的BM细胞凋亡，恢复T细胞免疫反应网络的平衡，恢复HSCs的造血功能。黄芪和当归的协同作用优于单独的每种药草。结论黄芪和当归可以干预T淋巴细胞的免疫平衡，抑制免疫攻击诱导的BM细胞凋亡，恢复T细胞免疫反应网络的平衡，恢复HSCs的造血功能。黄芪和当归的协同作用优于单独的每种药草。