This study aims to develop a liquid chromatography tandem-mass spectrometry (LC-MS/MS) method for vancomycin and creatinine measurement in dried blood spots (DBS) and to evaluate its clinical application. The analytes were extracted from DBS and analyzed by LC-MS/MS. Vancomycin and creatinine DBS and plasma concentrations were compared in 54 and 35 samples, respectively, from 29 patients. Accuracy was 94.4-102.6%, intra-assay precision was 2.1-5.6%, and inter-assay precision was 3.5-7.0%. Patients vancomycin plasma to DBS concentration ratios were highly variable (1.148 to 5.022), differently from creatinine (0.800 to 1.283). The assay has adequate analytical performance. Plasma concentrations can be satisfactorily predicted from DBS measurements for creatinine, but not for vancomycin, which limits its clinical application.

这项研究的目的是开发一种液相色谱串联质谱法（LC-MS / MS），用于测量干血斑（DBS）中的万古霉素和肌酐。并评估其临床应用。从DBS中提取分析物，并通过LC-MS / MS进行分析。对来自29例患者的54个和35个样本中的万古霉素和肌酐DBS和血浆浓度进行了比较。准确性为94.4-102.6％，批内准确性为2.1-5.6％，批间准确性为3.5-7.0％。患者万古霉素血浆与DBS的浓度比（肌酐）（0.800至1.283）差异很大（1.148至5.022）。该测定具有足够的分析性能。可以从DBS测量中满意地预测肌酐的血浆浓度，而万古霉素则不能，这限制了其临床应用。