Mobilization of reserves in germinated cereal grains is critical for early seedling vigour, global crop productivity, and hence food security. Gibberellins (GAs) are central to this process. We have developed a spatio-temporal model that describes the multifaceted mechanisms of GA regulation in germinated barley grain. The model was generated using RNA sequencing transcript data from tissues dissected from intact, germinated grain, which closely match measurements of GA hormones and their metabolites in those tissues. The data show that successful grain germination is underpinned by high concentrations of GA precursors in ungerminated grain, the use of independent metabolic pathways for the synthesis of several bioactive GAs during germination, and a capacity to abort bioactive GA biosynthesis. The most abundant bioactive form is GA₁, which is synthesized in the scutellum as a glycosyl conjugate that diffuses to the aleurone, where it stimulates de novo synthesis of a GA₃ conjugate and GA₄. Synthesis of bioactive GAs in the aleurone provides a mechanism that ensures the hormonal signal is relayed from the scutellum to the distal tip of the grain. The transcript data set of 33 421 genes used to define GA metabolism is available as a resource to analyse other physiological processes in germinated grain.

中文翻译：

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赤霉素在发芽大麦籽粒中的表达和含量的转录和生化分析。

动员发芽谷物中的储备物对于早期幼苗活力，全球作物生产力以及因此的粮食安全至关重要。赤霉素（GAs）在此过程中至关重要。我们已经开发了一个时空模型，该模型描述了发芽大麦籽粒中GA调控的多方面机制。该模型是使用从完整发芽谷物中解剖的组织中的RNA测序转录数据生成的，该数据与这些组织中GA激素及其代谢产物的测量值非常匹配。数据显示，未发芽谷物中高浓度的GA前体，发芽过程中使用独立的代谢途径合成多种生物活性GA以及中止生物活性GA生物合成的能力为成功的谷物发芽奠定了基础。最丰富的生物活性形式是GA₁，其在盾片中作为糖基缀合物合成，其扩散到糊粉中，在其中刺激GA ₃缀合物和GA _4的从头合成。糊粉中生物活性GA的合成提供了一种机制，可确保激素信号从盾片传递到谷物的远端。可用于定义GA代谢的33 421个基因的转录本数据集可作为分析发芽谷物中其他生理过程的资源。