BACKGROUND A rapid test system using fluorescent Mycobacterium terrae to evaluate the tuberculocidal efficacy of disinfectants has recently been published. Results were obtained in a significantly shorter time than was previously possible. AIM To compare the European Standard test system with the fluorescence assay and to validate the rapid test system, including particularly the quantitative suspension test. METHODS Quantitative suspension tests and quantitative carrier tests were carried out according to EN 14348 and EN 14563, respectively. Quantitative carrier tests and subsequent green fluorescent protein (GFP)-based determination of germicidal efficacy were carried out as described previously. A peracetic acid-based formulation was used as a test germicide. FINDINGS Testing of the germicide in the quantitative suspension test EN 14348 and in the quantitative carrier test EN 14563 revealed tuberculocidal efficacy at a concentration of 1% after 15 min contact time. Accordingly, data obtained from the fluorescence assay demonstrated that a germicide concentration of 1% was effective after 15 min, indicating no live mycobacteria following this treatment. Thus, identical in-use parameters for tuberculocidal efficacy were obtained either by applying the quantitative suspension and quantitative carrier tests EN 14348 and EN 14563 or by using the GFP-based rapid test system. CONCLUSION The GFP-based rapid test system compares well with the established European Standard test procedure including both phase 2, step 1 and phase 2, step 2 tests and provides a rapid and sensitive tool for testing germicides for relevant in-use concentrations and contact times.

中文翻译：

---

将针对结核杀菌功效的基于荧光的快速检测测定与EN 14348和EN 14563进行比较。

背景技术近来已经公开了一种快速测试系统，该系统使用土壤荧光分枝杆菌来评估消毒剂的杀结核功效。在比以前可能的时间短得多的时间内获得了结果。目的将欧洲标准测试系统与荧光分析法进行比较，并验证快速测试系统，特别是定量悬浮液测试。方法分别根据EN 14348和EN 14563进行了定量悬浮测试和定量载体测试。如前所述，进行了定量的载体测试以及随后基于绿色荧光蛋白（GFP）的杀菌功效的测定。基于过氧乙酸的制剂用作测试杀菌剂。结果在定量悬浮液测试EN 14348和定量载体测试EN 14563中对杀菌剂的测试表明，接触15分钟后，浓度为1％时，具有杀菌作用。因此，从荧光测定获得的数据表明，在15分钟后杀菌剂浓度为1％是有效的，表明该处理后没有活的分枝杆菌。因此，通过应用定量悬浮液和定量载体测试EN 14348和EN 14563或通过使用基于GFP的快速测试系统，可获得相同的结核杀菌功效使用参数。结论基于GFP的快速测试系统与既定的欧洲标准测试程序（包括第2阶段，第1阶段和第2阶段）相比具有很好的对比性