当前位置: X-MOL 学术Nat. Methods › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Localization microscopy at doubled precision with patterned illumination.
Nature Methods ( IF 36.1 ) Pub Date : 2019-12-09 , DOI: 10.1038/s41592-019-0657-7
Jelmer Cnossen 1, 2 , Taylor Hinsdale 1 , Rasmus Ø Thorsen 1 , Marijn Siemons 3 , Florian Schueder 4, 5 , Ralf Jungmann 4, 5 , Carlas S Smith 1, 2, 6 , Bernd Rieger 1 , Sjoerd Stallinga 1
Affiliation  

MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micrometer-sized field of view. We show a near two-fold improvement in precision over standard localization with the same photon count on DNA-origami nanostructures and tubulin in cells, using DNA-PAINT and STORM imaging.

中文翻译:

带有图案照明的双精度定位显微镜。

MINFLUX 在单分子定位精度方面取得了突破,但在视野方面受到限制。在这里,我们结合了传统宽场成像设置中的质心估计和照明模式引起的光子计数变化,以提取典型微米级视场的位置信息。我们使用 DNA-PAINT 和 STORM 成像显示,在 DNA 折纸纳米结构和细胞中的微管蛋白上具有相同光子计数的情况下,与标准定位相比,精度提高了近两倍。
更新日期:2019-12-11
down
wechat
bug