Gastric cancer (GC) is the third most common cause of cancer death worldwide, and the incidence of GC is higher in males than females. To investigate the gastric cellular response to hormone therapy, we developed a cell pseudotargeted metabolomics method based on liquid chromatography-hybrid triple quadrupole linear ion trap mass spectrometry (LC-QTRAP MS). Chromatographic separation, sample analysis and metabolite extraction were optimized in an integrated manner. The established pseudotargeted method, which combined nontargeted and targeted analyses, exhibited high selectivity, good repeatability and wide metabolome coverage. The method was then applied to discover differential metabolites from hormone-stimulated gastric cancer cells compared with the controls for the first time. The results demonstrated that hormone had subtle but phenotypically important alterations in nucleotide metabolism, amino acid metabolism, glycolysis, tricarboxylic acid (TCA) cycle, aminoacyl-tRNA biosynthesis and so on, which indicate that the developed method is a powerful tool for effective screening of endogenous polar metabolites in cell samples.

中文翻译：

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基于质谱的伪靶向代谢组学策略的开发，以分析激素刺激的胃癌细胞。

胃癌（GC）是全球第三大最常见的癌症死亡原因，男性中GC的发病率高于女性。为了研究胃细胞对激素治疗的反应，我们开发了一种基于液相色谱-混合三重四极杆线性离子阱质谱（LC-QTRAP MS）的细胞伪靶向代谢组学方法。整体上优化了色谱分离，样品分析和代谢物提取。建立的伪靶向方法结合了非靶向分析和靶向分析，具有较高的选择性，良好的重复性和广泛的代谢物覆盖范围。与第一次相比，该方法随后被用于从激素刺激的胃癌细胞中发现差异代谢产物。