IQ-domain GTPase-activating protein 1 (IQGAP1) is a multidomain scaffold protein that is involved in cytoskeleton dynamics and tumor metastasis. Although the role of IQGAP1 in various cancers had been reported, the function of IQGAP1 in pterygium has not been studied. In this study, surgically excised pterygium and control conjunctival tissue from cataract patients were analysed by immunohistochemistry, confocal microscopy, and Western blot for IQGAP1 expression, mast cell counts, and microvascular count. Pterygium was clinically divided into mild and severe types according to Tan's classification and Kim's criteria based on translucency and vascularity of the tissue. Greater clinical severity of pterygium was associated with higher expression of IQGAP1 expression. Compared to normal conjunctival tissue, severe pterygium had significantly higher IQGAP1 expression (P = 0.005), which strongly correlated to the number of microvessels (P = 0.003) and mast cells (P = 0.01). Confocal microscopy revealed IQGAP1 colocalization with mast cell and CD31. IQGAP1 expression was higher in the pterygium body compared to the head. In conclusion, the level of IQGAP1 expression was found to be correlated to the clinical severity of pterygium. Mast cells were identified in pterygium and is suspected to be involved in promoting fibrovascular invasion.

中文翻译：

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翼状severity肉严重程度与IQ域GTPase激活蛋白1（IQGAP1）和肥大细胞的相关性。

IQ域GTP酶激活蛋白1（IQGAP1）是涉及细胞骨架动力学和肿瘤转移的多域支架蛋白。尽管已经报道了IQGAP1在各种癌症中的作用，但尚未研究IQGAP1在翼状g肉中的功能。在这项研究中，通过免疫组织化学，共聚焦显微镜和Western blot分析了白内障患者的手术切除的翼状and肉和对照结膜组织的IQGAP1表达，肥大细胞计数和微血管计数。根据Tan的分类和基于组织的半透明性和血管性的Kim标准，翼状y肉在临床上分为轻度和重度两种类型。翼状clinical肉的临床严重程度更高与IQGAP1表达的更高表达有关。与正常结膜组织相比，重度翼状had肉具有较高的IQGAP1表达（P = 0.005），这与微血管的数量（P = 0.003）和肥大细胞的数量（P = 0.01）密切相关。共聚焦显微镜检查显示IQGAP1与肥大细胞和CD31共定位。与头部相比，翼状body肉中IQGAP1的表达更高。总之，发现IQGAP1表达水平与翼状the肉的临床严重程度相关。在翼状ery肉中鉴定出肥大细胞，并怀疑其与促进纤维血管浸润有关。发现IQGAP1表达水平与翼状the肉的临床严重程度相关。在翼状ery肉中鉴定出肥大细胞，并怀疑其与促进纤维血管浸润有关。发现IQGAP1表达水平与翼状the肉的临床严重程度相关。在翼状ery肉中鉴定出肥大细胞，并怀疑其与促进纤维血管浸润有关。