Group A rotavirus (RVA) is an important cause of diarrhoea in people, especially children, and animals globally. Due to the segmented nature of the RVA genome, animal RVA strains have the potential to adapt to the human host through reassortment with other co-infecting human viruses. Macaques share food and habitat with people, resulting in close interaction between these two species. This study aimed to detect and characterize RVA in rhesus macaques (Macaca mulatta) in Bangladesh. Faecal samples (N = 454) were collected from apparently healthy rhesus macaques from nine different sites in Bangladesh between February and March 2013. The samples were tested by one-step, real-time, reverse transcriptase-polymerase chain reaction (PCR). Four percent of samples (n = 20; 95% CI 2.7%-6.7%) were positive for RVA. RVA positive samples were further characterized by nucleotide sequence analysis of two structural protein gene fragments, VP4 (P genotype) and VP7 (G genotype). G3, G10, P[3] and P[15] genotypes were identified and were associated as G3P[3], G3P[15] and G10P[15]. The phylogenetic relationship between macaque RVA strains from this study and previously reported human strains indicates possible transmission between humans and macaques in Bangladesh. To our knowledge, this is the first report of detection and characterization of rotaviruses in rhesus macaques in Bangladesh. These data will not only aid in identifying viral sharing between macaques, human and other animals, but will also improve the development of mitigation measures for the prevention of future rotavirus outbreaks.

中文翻译：

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孟加拉恒河猴（Macaca mulatta）恒河猴的A群轮状病毒的分子特征。

轮状病毒A组轮状病毒（RVA）是导致人类（尤其是儿童）和动物腹泻的重要原因。由于RVA基因组的分段性质，动物RVA株具有通过与其他共同感染人类病毒重新分配而适应人类宿主的潜力。猕猴与人共享食物和栖息地，导致这两种物种之间的紧密互动。这项研究旨在检测和表征孟加拉恒河猴（猕猴）中的RVA。在2013年2月至3月之间，从孟加拉国9个不同地点的看似健康的恒河猴中采集了粪便样本（N = 454）。这些样本通过一步一步实时逆转录酶-聚合酶链反应（PCR）进行了测试。百分之四的样本（n = 20； 95％CI 2.7％-6.7％）的RVA呈阳性。通过对两个结构蛋白基因片段VP4（P基因型）和VP7（G基因型）的核苷酸序列分析进一步表征RVA阳性样品。确定了G3，G10，P [3]和P [15]基因型，并分别与G3P [3]，G3P [15]和G10P [15]相关。这项研究中的猕猴RVA毒株与先前报道的人类毒株之间的系统发育关系表明，孟加拉国人与猕猴之间可能存在传播。据我们所知，这是孟加拉国恒河猴中轮状病毒检测和鉴定的第一份报告。这些数据不仅有助于确定猕猴，人类和其他动物之间的病毒共享，而且还将改善缓解措施的发展，以预防未来的轮状病毒爆发。