Schwann cells (SC) are essential for the growth, maintenance, and regeneration of peripheral nerves, but the proteome of normal human SC is poorly defined. Here, a proteomic analysis by LC-MS/MS is performed to define the protein expression profile of primary human SC. A total of 19 557 unique peptides corresponding to 1553 individual proteins are identified. Ingenuity Pathway Analysis (IPA), Gene Ontology (GO), and Database for Annotation, Visualization, and Integrated Discovery (DAVID) are used to assign protein localization and function, and to define enriched pathways. EIF2, mTOR, and integrin signaling are among the most enriched pathways and the most enriched biological function is cell-cell adhesion, which is in agreement with the supportive role of SC in peripheral nerves. In addition, several nociceptors and synaptic proteins are identified and may contribute to the recently discovered role of SC in pain sensation and cancer progression. This proteome analysis of normal human SC constitutes a reference for future molecular explorations of physiological and pathological processes where SC are involved.

中文翻译：

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人类雪旺氏细胞的蛋白质组学概况。

雪旺细胞（SC）对于周围神经的生长，维持和再生至关重要，但是正常人SC的蛋白质组定义不明确。在这里，通过LC-MS / MS进行蛋白质组学分析，以定义原代人SC的蛋白质表达谱。鉴定出总共19 557个对应于1553个单个蛋白质的独特肽段。独创性途径分析（IPA），基因本体论（GO）和注释，可视化和综合发现数据库（DAVID）用于分配蛋白质定位和功能，并定义丰富的途径。EIF2，mTOR和整联蛋白信号传导是最丰富的途径之一，最丰富的生物学功能是细胞间粘附，这与SC在周围神经中的支持作用一致。此外，鉴定了几种伤害感受器和突触蛋白，它们可能有助于最近发现的SC在疼痛感和癌症进展中的作用。正常人SC的蛋白质组分析为将来涉及SC的生理和病理过程的分子探索提供了参考。