Full-Scale Osteochondral Regeneration by Sole Graft of Tissue-Engineered Hyaline Cartilage without Co-Engraftment of Subchondral Bone Substitute.,Advanced Healthcare Materials

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Full-Scale Osteochondral Regeneration by Sole Graft of Tissue-Engineered Hyaline Cartilage without Co-Engraftment of Subchondral Bone Substitute.
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2019-12-10 , DOI: 10.1002/adhm.201901304
Xiaolei Nie ₁ , Jian Yang _{1,

2} , Yon Jin Chuah ₁ , Wenzhen Zhu ₁ , Yvonne Peck ₁ , Pengfei He ₁ , Dong-An Wang _{1,

3}

Affiliation

In this study, full-scale osteochondral defects are hypothesized, which penetrate the articular cartilage layer and invade into subchondral bones, and can be fixed by sole graft of tissue-engineered hyaline cartilage without co-engraftment of any subchondral bone substitute. It is hypothesized that given a finely regenerated articular cartilage shielding on top, the restoration of subchondral bones can be fulfilled via spontaneous self-remodeling in situ. Hence, the key challenge of osteochondral regeneration lies in restoration of the non-self-regenerative articular cartilage. Here, traumatic osteochondral lesions to be repaired in rabbit knee models are endeavored using novel tissue-engineered hyaline-like cartilage grafts that are produced by 3D cultured porcine chondrocytes in vitro. Comparative trials are conducted in animal models that are implanted with living hyaline cartilage grafts (LhCG) and decellularized LhCG (dLhCG). Sound osteochondral regeneration is gradually revealed from both LhCG and dLhCG-implanted samples 50-100 d after implantation. Quality regeneration in both zones of articular cartilage and subchondral bones are validated by the restored osteochondral composition, structure, phenotype, and mechanical property, which validate the hypothesis of this study.

中文翻译：

通过组织工程透明软骨的单独移植进行全尺寸骨软骨再生，无需软骨下骨替代物的共同植入。

在这项研究中，假设了全面的骨软骨缺损，它穿透关节软骨层并侵入软骨下骨，并且可以通过组织工程透明软骨的单独移植物固定，而无需共同植入任何软骨下骨替代物。假设在顶部有一个精细再生的关节软骨屏蔽，软骨下骨的恢复可以通过原位自发的自我重塑来实现。因此，骨软骨再生的关键挑战在于恢复非自我再生的关节软骨。在这里，使用由体外 3D 培养的猪软骨细胞产生的新型组织工程透明样软骨移植物，尝试在兔膝模型中修复外伤性骨软骨损伤。比较试验是在植入了活的透明软骨移植物 (LhCG) 和脱细胞 LhCG (dLhCG) 的动物模型中进行的。植入后 50-100 天，从 LhCG 和 dLhCG 植入的样本中逐渐显示出良好的骨软骨再生。通过恢复的骨软骨组成、结构、表型和机械性能验证关节软骨和软骨下骨两个区域的质量再生，这验证了本研究的假设。

更新日期：2020-01-23

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