The CatSper channel is present and plays a key role in sperm motility of the Atlantic salmon (Salmo salar).,Comparative Biochemistry and Physiology A: Molecular & Integrative Physiology

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The CatSper channel is present and plays a key role in sperm motility of the Atlantic salmon (Salmo salar).
Comparative Biochemistry and Physiology A: Molecular & Integrative Physiology ( IF 2.1 ) Pub Date : 2019-12-10 , DOI: 10.1016/j.cbpa.2019.110634
Jorge Félix Beltrán Lissabet ₁ , Lisandra Herrera Belén ₂ , Manuel Lee-Estevez ₁ , Jennie Risopatrón ₃ , Iván Valdebenito ₄ , Elías Figueroa ₅ , Jorge G Farías ₁

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Among all the Ca2+ channels, CatSper channels have been one of the most studied in sperm of different species due to their demonstrated role in the fertilization process. In fish sperm, the calcium channel plays a key role in sperm activation. However, the functionality of the CatSper channels has not been studied in any of the fish species. For the first time, we studied the relationship of the CatSper channel with sperm motility in a fish, using Atlantic salmon (Salmo salar) as the model. The results of our study showed that the CatSper channel in Salmo salar has chemical-physical characteristics similar to those reported for mammalian CatSper channels. In this work, it was shown that Salmo salar CatSper 3 protein has a molecular weight of approximately 55-kDa similar to Homo sapiens CatSper 3. In silico analyses suggest that this channel forms a heterotetramer sensitive to the specific inhibitor HC-056456, with a binding site in the center of the pore of the CatSper channel, hindering or preventing the influx of Ca2+ ions. The in vitro assay of the sperm motility inhibition of Salmo salar with the inhibitor HC-056456 showed that sperm treated with this inhibitor significantly reduced the total and progressive motility (p < .0001), demonstrating the importance of this ionic channel for this cell. The complementation of the in silico and in vitro analyses of the present work demonstrates that the CatSper channel plays a key role in the regulation of sperm motility in Atlantic salmon.

中文翻译：

CatSper通道存在，并且在大西洋鲑（Salmo salar）的精子运动中起关键作用。

在所有Ca2 +通道中，由于CatSper通道在受精过程中发挥了重要作用，因此已成为对不同物种的精子中研究最多的通道之一。在鱼精子中，钙通道在精子激活中起关键作用。但是，尚未在任何鱼类中研究CatSper通道的功能。我们首次使用大西洋鲑（Salmo salar）作为模型，研究了CatSper通道与鱼类精子活力的关系。我们的研究结果表明，萨尔莫萨拉尔（Salmo salar）的CatSper通道具有类似于哺乳动物CatSper通道报道的化学-物理特征。在这项工作中，表明Salmo salar CatSper 3蛋白的分子量与智人CatSper 3相似，约为55-kDa。计算机分析表明，该通道形成了对特定抑制剂HC-056456敏感的异四聚体，其结合位点位于CatSper通道孔的中心，从而阻碍或阻止了Ca2 +离子的流入。用抑制剂HC-056456对Salmo salar的精子活动性抑制的体外分析表明，用该抑制剂处理的精子显着降低了总活动性和进行性活动性（p <.0001），表明该离子通道对该细胞的重要性。对本研究的计算机分析和体外分析的补充表明，CatSper通道在大西洋鲑精子活动性的调节中起关键作用。阻碍或阻止Ca2 +离子的流入。用抑制剂HC-056456对Salmo salar的精子活动性抑制的体外分析表明，用该抑制剂处理的精子显着降低了总活动性和进行性活动性（p <.0001），表明该离子通道对该细胞的重要性。对本研究的计算机分析和体外分析的补充表明，CatSper通道在大西洋鲑精子活动性的调节中起关键作用。阻碍或阻止Ca2 +离子的流入。用抑制剂HC-056456对Salmo salar的精子活动性抑制的体外分析表明，用该抑制剂处理的精子显着降低了总活动性和进行性活动性（p <.0001），表明该离子通道对该细胞的重要性。对本研究的计算机分析和体外分析的补充表明，CatSper通道在大西洋鲑精子活动性的调节中起关键作用。

更新日期：2019-12-11

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