Aqueous-Phase Synthesis of Hyaluronic Acid-Based Hydrogel Nanoparticles for Molecular Storage and Enzymatic Release,ACS Applied Polymer Materials

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Aqueous-Phase Synthesis of Hyaluronic Acid-Based Hydrogel Nanoparticles for Molecular Storage and Enzymatic Release
ACS Applied Polymer Materials ( IF 4.4 ) Pub Date : 2019-12-24 , DOI: 10.1021/acsapm.9b00834
Seungki Lee ₁ , Yunjeong Lee ₁ , Eun-Mi Kim ₂ , Kwang Woo Nam ₁ , Inhee Choi ₁

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Polysaccharide-based hydrogel particles have attracted attention as promising carriers for various biomolecules and drugs owing to their biocompatibility and biodegradability. The network structure and hydrophilic features of these hydrogels result in large surface area and high water uptake, which allows them to effectively store active molecules. However, conventional protocols for synthesizing such hydrogel nanoparticles usually require high amounts of organic solvents and cross-linkers, which may cause potential toxicity issues. Herein, we present a method for the water-phase synthesis of hyaluronic acid-based hydrogel particles (HAGs) and their application for safely encapsulating biomolecules. By controlling the synthesis procedures and ingredients, the size and shape of the HAGs can be systematically optimized. The colloidal stability of the produced HAGs is evaluated by measuring their light-scattering properties under various conditions. The activity of loaded molecules, such as small antioxidants and large proteins, is also monitored via activity assays. Finally, controlled release of the loaded molecules is achieved via the enzymatic degradation of hyaluronic acid. We believe that the proposed HAGs could be used as promising carriers for protecting active ingredients from the outer environment to further develop biomedical and cosmetic products.

中文翻译：

基于透明质酸的水凝胶纳米粒子的分子合成和酶促释放的水相合成

基于多糖的水凝胶颗粒因其生物相容性和生物降解性而作为各种生物分子和药物的有前途的载体而受到关注。这些水凝胶的网络结构和亲水特性导致较大的表面积和较高的吸水率，这使它们可以有效地存储活性分子。然而，用于合成这种水凝胶纳米颗粒的常规方案通常需要大量的有机溶剂和交联剂，这可能引起潜在的毒性问题。在这里，我们提出了一种透明质酸基水凝胶颗粒（HAGs）的水相合成方法及其在安全封装生物分子中的应用。通过控制合成步骤和成分，可以系统地优化HAG的大小和形状。通过在各种条件下测量其光散射特性来评估所产生的HAG的胶体稳定性。还可以通过活性测定法来监测负载的分子（例如小抗氧化剂和大蛋白）的活性。最后，通过透明质酸的酶促降解实现了负载分子的受控释放。我们认为，提出的HAG可用作保护活性成分不受外界环境影响的有前途的载体，以进一步开发生物医学和化妆品产品。通过透明质酸的酶促降解可实现负载分子的受控释放。我们认为，提出的HAG可用作保护活性成分不受外界环境影响的有前途的载体，以进一步开发生物医学和化妆品产品。通过透明质酸的酶促降解可实现负载分子的受控释放。我们认为，提出的HAG可用作保护活性成分不受外界环境影响的有前途的载体，以进一步开发生物医学和化妆品产品。

更新日期：2019-12-25

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