Ultraviolet radiations (UVR) are responsible for a wide variety of acute and chronic effects on the animal skin. However, the effect of UVR-induced oxidative stress and protection through paracrine factors on animal skin has received little attention. We previously demonstrated how heat stress-induced adaptation in Bos indicus melanocytes was dependent on the level of melanin and reduction of apoptosis. Therefore, in the present investigation, the survival mechanisms adopted by melanocytes under UV stress and the role of α-MSH in cell survival under in vitro conditions were studied. After the treatment of melanocyte cells with UVR (using Osram ultravitalux 300 W lamp), analysis of Gene expression using Real-Time PCR was done to study the adopted molecular pathways under stressful conditions. In addition, α-MSH was used to assess its modulating role in cell survival under stress. This study revealed the increase in the expression of genes related to melanogenesis, cell cycle, heat shock proteins, and apoptosis of the cells after UVR stress and demonstrated the role of paracrine factor (α-MSH) in elevating the protection response to stressful conditions like UVR stress by increasing the melanogenesis and decreasing the mitochondrial-mediated apoptosis. Based on the results of the present study, it can be stated that α-MSH can play a pivotal role in the protection of animal skin cells under stressful conditions in climate-changing scenario.

中文翻译：

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α-黑素细胞刺激激素（α-MSH）在调节UVR胁迫下印度s黑素细胞采取的分子机制中的作用。

紫外线（UVR）对动物皮肤产生多种急性和慢性影响。但是，UVR诱导的氧化应激和旁分泌因子对动物皮肤的保护作用几乎没有引起注意。我们以前证明了热应激诱导的Bos indicus黑色素细胞适应如何取决于黑色素的水平和凋亡的减少。因此，在本研究中，研究了紫外线条件下黑素细胞采用的存活机制以及α-MSH在体外条件下细胞存活中的作用。用UVR处理黑色素细胞后（使用Osram ultravitalux 300 W灯），使用实时PCR对基因表达进行分析，以研究在压力条件下采用的分子途径。此外，α-MSH被用来评估其在应激条件下对细胞存活的调节作用。这项研究揭示了UVR应激后与黑色素生成，细胞周期，热休克蛋白和细胞凋亡相关的基因表达增加，并证明了旁分泌因子（α-MSH）在增强对诸如应激等应激性疾病的保护反应中的作用。通过增加黑色素生成和减少线粒体介导的细胞凋亡来增强UVR压力。根据本研究的结果，可以说在气候变化的情况下，α-MSH在压力条件下对动物皮肤细胞的保护中起着关键作用。UVR应激后细胞的凋亡和凋亡，并证明旁分泌因子（α-MSH）在通过增加黑色素生成和减少线粒体介导的凋亡而增强对诸如UVR应激等应激条件的保护反应中的作用。根据本研究的结果，可以说在气候变化的情况下，α-MSH在压力条件下对动物皮肤细胞的保护中起着关键作用。UVR应激后细胞的凋亡和凋亡，并证明旁分泌因子（α-MSH）在通过增加黑色素生成和减少线粒体介导的凋亡而增强对诸如UVR应激等应激条件的保护反应中的作用。根据本研究的结果，可以说在气候变化的情况下，α-MSH在压力条件下对动物皮肤细胞的保护中起着关键作用。