Cordyceps cicadae is a well-known traditional Chinese medicine for treating palpitations and eye diseases. It contains several bioactive compounds such as adenosine, N6-(2-hydroxyethyl)-adenosine (HEA), and polysaccharide. Those bioactive compounds have been reported to perform anti-oxidation and anti-inflammatory properties and provide renal protection. In this study, we researched different fermentation conditions in order to enhance the biomass, adenosine, HEA, and polysaccharide productions of C. cicadae NTTU 868. Solid fermentation was carried out with different grain substrates (barley, oat, rice and wheat). Various submerged fermentation scales were used to produce the C. cicadae NTTU 868 mycelium. The results of solid fermentation revealed that C. cicadae NTTU 868 produced higher adenosine and HEA concentrations in oat rather than in other substrates. C. cicadae NTTU 868 mycelium had obtained the highest concentrations of adenosine and HEA on Day 2 as using the small-scale submerged fermentation. Furthermore, potato dextrose broth with extra 0.2% of yeast extract was able to result in higher HEA concentration. In conclusion, using submerged fermentation to culture C. cicadae NTTU 868 resulted in more efficient adenosine, HEA, and polysaccharide productions than using solid-fermentation, especially when 0.2% of yeast extract was used in the PDB. Importantly, this can be easily scaled-up in the fermentation industry.

中文翻译：

---

使用水下发酵快速增加虫草蝉NTTU 868的N6-（2-羟乙基）-腺苷，腺苷和多糖的产量。

虫草蝉是治疗心pit和眼疾的著名中药。它包含几种生物活性化合物，例如腺苷，N6-（2-羟乙基）-腺苷（HEA）和多糖。据报道，那些生物活性化合物具有抗氧化和抗炎特性，并能提供肾脏保护作用。在这项研究中，我们研究了不同的发酵条件以提高蝉蝉NTTU 868的生物量，腺苷，HEA和多糖产量。固体发酵是在不同的谷物底物（大麦，燕麦，大米和小麦）下进行的。各种淹没式发酵规模用于生产蝉蝉NTTU 868菌丝体。固体发酵的结果表明蝉科NTTU 868在燕麦中而不是其他底物中产生更高的腺苷和HEA浓度。蝉蝉NTTU 868菌丝体在第2天使用小规模的水下发酵获得了最高浓度的腺苷和HEA。此外，马铃薯右旋糖肉汤中添加了0.2％的酵母提取物能够提高HEA浓度。总之，使用沉浸式发酵培养蝉蝉NTTU 868比使用固体发酵更有效地生产腺苷，HEA和多糖，特别是在PDB中使用0.2％的酵母提取物时。重要的是，这很容易在发酵工业中扩大规模。