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Identification of increased expression of activating Fc receptors and novel findings regarding distinct IgE and IgM receptors in Kawasaki disease
Pediatric Research ( IF 3.1 ) Pub Date : 2019-12-09 , DOI: 10.1038/s41390-019-0707-y
Ling-Sai Chang , Mindy Ming-Huey Guo , Mao-Hung Lo , Ho-Chang Kuo

Kawasaki disease (KD) is associated with expression and methylation of Fc gamma receptor genes. We characterized immunoglobulin A (IgA), IgE, IgG, and IgM receptor expression levels in KD. Fc receptor expression levels were characterized using GeneChip Human Transcriptome Array 2.0 (HTA 2.0) with 18 KD patients, 18 non-febrile controls, and 18 febrile controls. Another 48 control individuals and 46 patients with KD were measured using pyrosequencing for the methylation levels. The mRNA expression levels of FCER1A and FCER2 were significantly lower in KD patients than in non-febrile controls and then rose following treatments with intravenous immunoglobulin (IVIG). Expression levels of FCER1G increased compared to the non-febrile subjects and then subsided after IVIG. FCER1A methylation was significantly lower among KD patients and even lower in KD patients with IVIG resistance. HTA analysis revealed higher mRNA levels of FCAR, FCGR1C, and FCGR2A in KD patients. FCMR mRNA expression levels were significantly lower in KD patients. FCMR expression levels rose after IVIG treatment. After IVIG, FCGR1A, B, and C decreased even lower than the febrile controls. This is the first study indicating that IgA, IgE, IgG, and IgM receptors are associated with KD. We highlighted potential biomarkers related to Fc receptors and their regulation.

中文翻译:

川崎病中激活 Fc 受体表达增加的鉴定以及关于不同 IgE 和 IgM 受体的新发现

川崎病 (KD) 与 Fc γ 受体基因的表达和甲基化有关。我们表征了 KD 中免疫球蛋白 A (IgA)、IgE、IgG 和 IgM 受体的表达水平。使用 GeneChip Human Transcriptome Array 2.0 (HTA 2.0) 对 18 名 KD 患者、18 名非发热对照和 18 例发热对照表征 Fc 受体表达水平。使用焦磷酸测序测量另外 48 名对照个体和 46 名 KD 患者的甲基化水平。KD 患者 FCER1A 和 FCER2 的 mRNA 表达水平显着低于非发热对照组,然后在静脉注射免疫球蛋白 (IVIG) 治疗后升高。与不发热的受试者相比,FCER1G 的表达水平增加,然后在 IVIG 后消退。FCER1A 甲基化在 KD 患者中显着较低,在具有 IVIG 耐药性的 KD 患者中甚至更低。HTA 分析显示 KD 患者中 FCAR、FCGR1C 和 FCGR2A 的 mRNA 水平较高。KD 患者的 FCMR mRNA 表达水平显着降低。IVIG 治疗后 FCMR 表达水平上升。IVIG 后,FCGR1A、B 和 C 的下降甚至低于发热对照组。这是第一项表明 IgA、IgE、IgG 和 IgM 受体与 KD 相关的研究。我们强调了与 Fc 受体及其调节相关的潜在生物标志物。和 C 的下降甚至低于发热对照组。这是第一项表明 IgA、IgE、IgG 和 IgM 受体与 KD 相关的研究。我们强调了与 Fc 受体及其调节相关的潜在生物标志物。和 C 的下降甚至低于发热对照组。这是第一项表明 IgA、IgE、IgG 和 IgM 受体与 KD 相关的研究。我们强调了与 Fc 受体及其调节相关的潜在生物标志物。
更新日期:2019-12-09
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