Rhamnolipids are widely investigated to control the growth of food pathogenic bacteria as well as repair environmental problems. This work was proposed to in situ recover rhamnolipids from the fermentation medium of Pseudomonas aeruginosa D1 through foam fractionation. The kinetic model of the fermentation process of Pseudomonas aeruginosa was established, solved and tested. The model fitted well with the experimental data. The results showed that the initial addition rhamnolipids concentration of 1.25 g/L had little effect on the growth of bacteria, but could significantly affect the synthesis of rhamnolipids. Under the suitable conditions of volumetric air flow rate 60 mL/min, loading liquid volume 100 mL, pore diameter of gas distributor 160 μm, the enrichment ratio and the recovery percentage of rhamnolipids reached 4.83 and 75.15%, respectively. Experimental results indicated that the maximum yield of rhamnolipids could be obtained by coupling foam fractionation with fermentation at 42 h, in which the synthesis rate of rhamnolipids was maximal. Moreover, the yield of rhamnolipids per unit volume of fermentation medium was 16.46 g/L, and the yield was 32.63% higher than that of uncoupled operation, and 13.04 and 10.32% higher than those of coupled operation at other fermentation times.

鼠李糖脂已被广泛研究以控制食物致病细菌的生长以及修复环境问题。提出这项工作是通过泡沫分离从铜绿假单胞菌D1的发酵培养基中原位回收鼠李糖脂。铜绿假单胞菌发酵过程动力学模型建立，解决和测试。该模型与实验数据吻合得很好。结果表明，初始添加鼠李糖脂浓度为1.25 g / L对细菌的生长影响不大，但可以显着影响鼠李糖脂的合成。在风量为60mL / min，装液量为100mL，气体分布器的孔径为160μm的合适条件下，鼠李糖脂的富集率和回收率分别达到4.83和75.15％。实验结果表明，通过分馏泡沫分馏与42 h发酵，可获得鼠李糖脂的最大产量，其中鼠李糖脂的合成速率最大。另外，每单位体积的发酵培养基鼠李糖脂的产量为16.46g / L，产量为32。