Demands for anthocyanins are steadily growing due to their positive bioactivities in human health and food coloration. This study was performed to evaluate the characteristics of purified purple passion fruit epicarp anthocyanins (PFEA) as well as its anti-fatigue activity and underlying mechanisms in mice. The result showed that three major anthocyanins including cyanidin-3-O-glucoside, cyanidin-3-O-rutinoside and peonidin-3-O-glucoside were identified, and the total content of these anthocyanins was 710.40 mg/g in PFEA. PFEA significantly prolonged the weight-loaded forced swimming time, decreased lactate dehydrogenase (LDH), the blood lactate (BLA) and the blood urea nitrogen (BUN) levels, and increased the liver glycogen (LG) content. Meanwhile, PFEA could ameliorate fluctuates in oxidative stress biomarkers (MDA), antioxidant enzymes (SOD) and inflammatory cytokines (TNF-α, IL-1β, IL-6). Moreover, the mRNA expression of PGC-1α and PPARα in skeletal muscles were also up-regulated by PFEA treatment. In conclusion, these results showed that supplementing PFEA could relieve fatigue effects.

由于花青素在人类健康和食物着色中具有积极的生物活性，因此对花青素的需求正在稳定增长。进行这项研究以评估纯化的紫色西番莲果果皮花青素（PFEA）的特性，以及其抗疲劳活性及其在小鼠体内的潜在机制。结果表明，已鉴定出花青素-3-O-葡糖苷，花青素-3-O-芸香苷和peonidin-3-O-葡糖苷三种主要花色苷，在PFEA中这些花色苷的总含量为710.40 mg / g。PFEA显着延长了负重游泳的时间，降低了乳酸脱氢酶（LDH），血液乳酸（BLA）和血液尿素氮（BUN）的水平，并增加了肝糖原（LG）的含量。同时，PFEA可以减轻氧化应激生物标志物（MDA）的波动，抗氧化剂（SOD）和炎性细胞因子（TNF-α，IL-1β，IL-6）。此外，PFEA处理还可以上调骨骼肌中PGC-1α和PPARα的mRNA表达。总之，这些结果表明，补充PFEA可以缓解疲劳影响。