In the year of 2019, we celebrate the centenary of the publication of the remarkable paper by Otto Stern and Max Volmer concerning the kinetics analysis of fluorescence quenching. Their achievement changed enormously many fields of science with great impact in the studies of electronic spectroscopy and kinetics of organic, inorganic and biological compounds in condensed phase. The development of molecular photochemistry was in great part based on the use of the Stern-Volmer (SV) approach to investigate bimolecular interaction in the electronic excited-state. This paper reviews and summarizes the assumptions behind the Stern-Volmer equation and the extensions of the theory to other important situations. Discussions about the use of the SV approach to investigate probe and quencher distribution, association, diffusion and reaction at the molecular level obtained from advanced fluorescence microscopy methods are also included.

在2019年，我们庆祝Otto Stern和Max Volmer发表了有关荧光猝灭动力学分析的杰出论文的百年纪念。他们的成就极大地改变了许多科学领域，对电子光谱学以及凝聚相中有机，无机和生物化合物的动力学产生了重大影响。分子光化学的发展很大程度上是基于使用Stern-Volmer（SV）方法研究电子激发态下的双分子相互作用。本文回顾并总结了Stern-Volmer方程背后的假设以及该理论在其他重要情况下的扩展。关于使用SV方法研究探针和淬灭剂分布，缔合，