Identification of soybean drought-tolerant genotypes and loci correlated with agronomic traits contributes new candidate genes for breeding.,Plant Molecular Biology

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Identification of soybean drought-tolerant genotypes and loci correlated with agronomic traits contributes new candidate genes for breeding.
Plant Molecular Biology ( IF 3.9 ) Pub Date : 2019-12-09 , DOI: 10.1007/s11103-019-00934-7
Limiao Chen _{1,

2} , Yisheng Fang _{1,

2} , Xiangyong Li ₃ , Kai Zeng ₄ , Haifeng Chen _{1,

2} , Hengbin Zhang ₄ , Hongli Yang _{1,

2} , Dong Cao _{1,

2} , Qingnan Hao _{1,

2} , Songli Yuan _{1,

2} , Chanjuan Zhang _{1,

2} , Wei Guo _{1,

2} , Shuilian Chen _{1,

2} , Zhonglu Yang _{1,

2} , Zhihui Shan _{1,

2} , Xiaojuan Zhang _{1,

2} , Dezhen Qiu _{1,

2} , Yong Zhan ₄ , Xin-An Zhou _{1,

2}

Affiliation

Drought tolerance level of 136 soybean genotypes, the correlations among traits were evaluated, and several important drought-tolerant genotypes, traits, SNPs and genes were possibly useful for soybean genetic breeding. Drought is an adverse environmental factor affecting crops growth, development, and yield. Promising genotypes and genes with improved tolerance to drought are probably effective ways to alleviate the situation. In this study, our main task was to determine drought tolerance level of 136 soybean genotypes, the correlations among physiological and agronomic traits under drought, and drought-tolerant single nucleotide polymorphism (SNPs) and genes. In this study, twenty-six varieties were identified as excellent tolerant genotypes to stress among which S14, S93 and S135 with high drought-tolerant index (DTI > 1.3) and yield (Y > 300 kg). Fourteen varieties were identified as drought-sensitive genotypes, such as S25, S45 and S58, with low drought-tolerant index (DTI < 0.5). 422 SNPs and 302 genes correlated with seed number per plant (SNPP), maturity (M), number of seeds per pod (NSPP), node number of main stem (NNMS), Stem diameter (SD) and pull stem (PS) were detected under well-watered and drought conditions by genome-wide association study (GWAS). Among them, we found SNPs (Chr 3:1758920-1958934) between drought-tolerant and sensitive genotypes. 13 genes (Glyma.03G017800, Glyma.03G018000, Glyma.03G018200, Glyma.03G018400, Glyma.03G018500, Glyma.03G018600, Glyma.03G018700, Glyma.03G018800, Glyma.03G018900, Glyma.03G019000, Glyma.03G019100, Glyma.03G019200, Glyma.03G019300) correlated with NNMS were detected. By qRT-PCR, the expression level of Glyma.03G018000 and Glyma.03G018900 in drought-tolerant varieties was significantly increased, but low or no expression in sensitive varieties under drought stress. This study provides important drought-tolerant genotypes, traits, SNPs and potential genes, possibly useful for soybean genetic breeding.

中文翻译：

大豆耐旱基因型和与农艺性状相关的基因座的鉴定为育种提供了新的候选基因。

对136个大豆基因型的耐旱水平以及性状之间的相关性进行了评价，发现了一些重要的耐旱基因型、性状、SNP和基因，可能对大豆遗传育种有用。干旱是影响农作物生长、发育和产量的不利环境因素。有前途的基因型和具有改善干旱耐受性的基因可能是缓解这种情况的有效方法。本研究的主要任务是确定136个大豆基因型的耐旱水平、干旱条件下生理农艺性状的相关性以及耐旱单核苷酸多态性（SNP）和基因。本研究鉴定出26个优良耐逆基因型，其中S14、S93和S135具有较高的耐旱指数（DTI > 1.3）和产量（Y > 300 kg）。鉴定出S25、S45、S58等14个干旱敏感基因型，耐旱指数较低（DTI<0.5）。与单株种子数（SNPP）、成熟度（M）、每荚种子数（NSPP）、主茎节数（NNMS）、茎直径（SD）和拉茎（PS）相关的422个SNP和302个基因通过全基因组关联研究（GWAS）在浇水良好和干旱条件下检测到。其中，我们在耐旱和敏感基因型之间发现了SNP（Chr 3：1758920-1958934）。 13个基因（Glyma.03G017800、Glyma.03G018000、Glyma.03G018200、Glyma.03G018400、Glyma.03G018500、Glyma.03G018600、Glyma.03G018700、Glyma.03G018800、 G018900、格利马.03G019000、格利马.03G019100、格利马.03G019200 , Glyma.03G019300) 与 NNMS 相关。通过qRT-PCR检测Glyma.03G018000和Glyma的表达水平。03G018900在耐旱品种中显着表达，但在干旱胁迫敏感品种中低表达或无表达。这项研究提供了重要的耐旱基因型、性状、SNP 和潜在基因，可能对大豆遗传育种有用。

更新日期：2019-12-09

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