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Probing Structure and Reaction Dynamics of Proteins Using Time-Resolved Resonance Raman Spectroscopy
Chemical Reviews ( IF 51.4 ) Pub Date : 2019-12-09 , DOI: 10.1021/acs.chemrev.9b00429
David Buhrke 1 , Peter Hildebrandt 1
Affiliation  

The mechanistic understanding of protein functions requires insight into the structural and reaction dynamics. To elucidate these processes, a variety of experimental approaches are employed. Among them, time-resolved (TR) resonance Raman (RR) is a particularly versatile tool to probe processes of proteins harboring cofactors with electronic transitions in the visible range, such as retinal or heme proteins. TR RR spectroscopy offers the advantage of simultaneously providing molecular structure and kinetic information. The various TR RR spectroscopic methods can cover a wide dynamic range down to the femtosecond time regime and have been employed in monitoring photoinduced reaction cascades, ligand binding and dissociation, electron transfer, enzymatic reactions, and protein un- and refolding. In this account, we review the achievements of TR RR spectroscopy of nearly 50 years of research in this field, which also illustrates how the role of TR RR spectroscopy in molecular life science has changed from the beginning until now. We outline the various methodological approaches and developments and point out current limitations and potential perspectives.

中文翻译:

使用时间分辨共振拉曼光谱探测蛋白质的结构和反应动力学

对蛋白质功能的机械理解需要深入了解结构和反应动力学。为了阐明这些过程,采用了各种实验方法。其中,时间分辨(TR)共振拉曼(RR)是一种特别通用的工具,用于探测带有辅因子的蛋白过程,这些蛋白在可见范围内具有电子跃迁,例如视网膜或血红素蛋白。TR RR光谱法具有同时提供分子结构和动力学信息的优势。各种TR RR光谱方法可涵盖直至飞秒时间范围的宽动态范围,并已用于监测光诱导的反应级联,配体结合和解离,电子转移,酶促反应以及蛋白质解折叠和重折叠。在这个帐户中,我们回顾了TR RR光谱学在该领域近50年研究的成就,这也说明了TR RR光谱学在分子生命科学中的作用从开始到现在是如何变化的。我们概述了各种方法论方法和发展,并指出了当前的局限性和潜在的观点。
更新日期:2019-12-09
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