Docosahexaenoic acid (DHA)‐rich diacylglycerol (DAG)‐rich oil was prepared by lipase‐catalyzed glycerolysis of microbial oil from Schizochytrium sp. in a solvent‐free system. The reaction parameters including lipase type, substrate molar ratio, temperature, lipase concentration, and reaction time were screened. The selected conditions were determined as follows: Novozym® 435 (Novozymes A/S, Bagsvaerd, Denmark) as biocatalyst at 8 wt%, substrate ratio (DHA‐rich microbial TAG/glycerol) of 1:1 mol/mol, temperature of 50 °C, and reaction time of 12 hours. Under these conditions, the triacylglycerol (TAG), DAG, and monoacylglycerol (MAG) contents in the product were 36.4%, 48.2%, and 15.4%, respectively. The lipase was reused successively for 18 cycles without significant loss of activity under the conditions given above. Fatty acid composition analysis of the final product showed that the contents of DHA in TAG, DAG, and MAG were 53.9%, 44.9%, and 34.8%, respectively. DHA‐rich DAG has the potential to be used as an ingredient in infant formula to increase the bioavailability of DHA.

中文翻译：

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脂肪酶催化裂殖壶菌属微生物油的甘油糖分解制备二十二碳六烯酸-富二酰基甘油-富油。在无溶剂系统中

富含二十二碳六烯酸（DHA）的富含二酰基甘油（DAG）的油是通过脂肪酶催化的裂殖壶菌微生物油的甘油分解制备的sp。在无溶剂系统中。筛选了包括脂肪酶类型，底物摩尔比，温度，脂肪酶浓度和反应时间在内的反应参数。选择的条件如下：435（生物酶A / S，Bagsvaerd，丹麦）作为生物催化剂，含量为8 wt％，底物比例（富含DHA的微生物TAG /甘油）为1：1 mol / mol，温度为50 ℃，反应时间为12小时。在这些条件下，产物中的三酰基甘油（TAG），DAG和单酰基甘油（MAG）的含量分别为36.4％，48.2％和15.4％。在上述条件下，脂肪酶连续重复使用18个循环而没有明显的活性损失。最终产物的脂肪酸组成分析表明，TAG，DAG和MAG中DHA的含量分别为53.9％，44.9％和34.8％。