Abstract We endeavoured to probe into and compare the possible effect(s) of different extraction techniques (accelerated solvent extraction (ASE), microwave-assisted extraction (MAE), ultrasonication-assisted extraction (UAE), maceration, and Soxhlet extraction (SE)) on the bioactivity (antioxidant and enzyme inhibitory activities) of the aerial parts of Helichrysum stoechas subsp. barrelieri (Ten.) Nyman. Total phenolic and flavonoid contents of the extracts obtained by different extraction methods followed the order of ASE > MAE > UAE > maceration > SE. Extract obtained by ASE was the most potent radical scavenger (219.92 and 313.12 mg Trolox equivalent [TE]/g, against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), respectively) and reducing agent (927.39 and 662.87 mg TE/g, for cupric reducing antioxidant capacity (CUPRAC) and ferric reducing antioxidant power (FRAP), respectively). Helichrysum stoechas extract obtained by UAE (18.67 mg ethylenediaminetetraacetic equivalent [EDTAE]/g) was the most active metal chelator and inhibitor of acetylcholinesterase (4.23 mg galantamine equivalent [GALAE]/g) and butyrylcholinesterase (6.05 mg GALAE/g) cholinesterase. Extract from maceration (183.32 mg kojic acid equivalent [KAE]/g) was most active against tyrosinase while ASE extract (1.66 mmol acarbose equivalent [ACAE]/g) effectively inhibited α-glucosidase. In conclusion, data amassed herein tend to advocate for the use of non-conventional extraction techniques, namely ASE and UAE, for the extraction of bioactive secondary metabolites from H. stoechas aerial parts.

中文翻译：

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蜡菊亚种植物化学特征和生物制药潜力的比较探索。通过五种提取技术获得的桶状提取物

摘要 我们努力探索和比较不同提取技术（加速溶剂提取 (ASE)、微波辅助提取 (MAE)、超声辅助提取 (UAE)、浸渍和索氏提取 (SE)）可能产生的影响。 ) 对 Helichrysum stoechas subsp. 地上部分的生物活性（抗氧化和酶抑制活性）的影响。巴里耶里（十）尼曼。不同提取方法提取物的总酚和总黄酮含量顺序为ASE>MAE>UAE>浸渍>SE。通过 ASE 获得的提取物是最有效的自由基清除剂（219.92 和 313.12 mg Trolox 当量 [TE]/g，针对 2,2-二苯基-1-苦基肼 (DPPH) 和 2,2'-叠氮基-双（3-乙基苯并噻唑啉）- 6-磺酸（ABTS））和还原剂（927.39 和 662.87 mg TE/g，分别为铜还原抗氧化能力 (CUPRAC) 和铁还原抗氧化能力 (FRAP)。阿联酋获得的蜡菊提取物（18.67 毫克乙二胺四乙酸当量 [EDTAE]/g）是最活跃的金属螯合剂和乙酰胆碱酯酶抑制剂（4.23 毫克雪花胺当量 [GALAE]/g）和丁酰胆碱酯酶（6.05 毫克 GALAE/g）胆碱酯酶。浸渍提取物 (183.32 mg 曲酸当量 [KAE]/g) 对酪氨酸酶最有效，而 ASE 提取物 (1.66 mmol 阿卡波糖当量 [ACAE]/g) 有效抑制 α-葡萄糖苷酶。总之，本文收集的数据倾向于提倡使用非常规提取技术，即 ASE 和阿联酋，从 H. stoechas 地上部分提取生物活性次级代谢物。