Structured surfaces with both anti-bacterial capability and biocompatibility are essential to medical devices including orthopedic implants. Over the past few years, numerous studies indicated that nanostructures such as nanotubes, nanowires, nano-pillars and nano-ripples were capable of repelling or killing bacteria, while the morphology and dimensions of the nanostructures showed essential influence on the adhesion and proliferation of cells. The long-term functionalities of implants depend on their reliable anti-bacterial performance at the same time enhanced biocompatibility. However, researches covering both these two aspects are still very limited. In this paper, we applied one-step femtosecond laser irradiation to produce three types of nano-ripples, and to investigate their anti-bacterial behavior and their biocompatibility. Our results demonstrate that all the three types of nano-ripples are capable of preventing bacterial colonization and biofilm formation, with their anti-bacteria rates towards E. coli respectively 43%, 49% and 56%. Meanwhile, the femtosecond laser induced nano-ripples exhibit good rat mesenchymal stem cells (MSCs) proliferation capacity and spreading performance, and the nano-ripples affect significantly the orientation of MSCs. The results offer valuable information and potentials on the anti-bacteria and biocompatibility of nanostructures.

具有抗菌功能和生物相容性的结构化表面对于包括整形外科植入物的医疗设备至关重要。在过去的几年中，大量研究表明，纳米结构（例如纳米管，纳米线，纳米柱和纳米波纹）能够排斥或杀死细菌，而纳米结构的形态和尺寸则对细胞的粘附和增殖具有至关重要的影响。 。植入物的长期功能取决于其可靠的抗菌性能，同时增强了生物相容性。但是，涵盖这两个方面的研究仍然非常有限。在本文中，我们应用了一步飞秒激光辐照来产生三种类型的纳米波纹，并研究了它们的抗菌性能和生物相容性。大肠杆菌分别为43％，49％和56％。同时，飞秒激光诱导的纳米波纹具有良好的大鼠间充质干细胞（MSCs）的增殖能力和扩散性能，并且纳米波纹对MSCs的取向有显着影响。结果为纳米结构的抗菌和生物相容性提供了有价值的信息和潜力。