Zika virus (ZIKV) nonstructural protein 5 (NS5) plays a critical role in viral RNA replication and mediates key virus-host cell interactions. As with other flavivirus NS5 proteins, ZIKV NS5 is primarily found in the nucleus. We previously reported that the NS5 protein of dengue virus, another flavivirus, localized to centrosomes during cell division. Here we show that ZIKV NS5 also relocalizes from the nucleus to centrosomes during mitosis. In infected cells with supernumerary centrosomes, NS5 was present at all centrosomes. Transient expression of NS5 in uninfected cells confirmed that centrosomal localization was independent of other viral proteins. Live-cell imaging demonstrated that NS5-GFP accumulated at centrosomes shortly after break down of nuclear membrane and remained there through mitosis. Cells expressing NS5-GFP took longer to complete mitosis than control cells. Finally, an analysis of ZIKV NS5 binding partners revealed several centrosomal proteins, providing potential direct links between NS5 and centrosomes.

中文翻译：

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寨卡病毒 NS5 在细胞分裂过程中定位于中心体。

寨卡病毒 (ZIKV) 非结构蛋白 5 (NS5) 在病毒 RNA 复制中起关键作用，并介导关键的病毒-宿主细胞相互作用。与其他黄病毒 NS5 蛋白一样，ZIKV NS5 主要存在于细胞核中。我们之前曾报道登革热病毒（另一种黄病毒）的 NS5 蛋白在细胞分裂过程中定位于中心体。在这里，我们表明 ZIKV NS5 在有丝分裂期间也会从细胞核重新定位到中心体。在具有多余中心体的感染细胞中，NS5 存在于所有中心体中。NS5 在未感染细胞中的瞬时表达证实了中心体定位与其他病毒蛋白无关。活细胞成像表明 NS5-GFP 在核膜破裂后不久在中心体积累并通过有丝分裂保持在那里。与对照细胞相比，表达 NS5-GFP 的细胞完成有丝分裂所需的时间更长。最后，对 ZIKV NS5 结合伙伴的分析揭示了几种中心体蛋白，为 NS5 和中心体之间提供了潜在的直接联系。