Deacidification of high-acid soy sauce residue (SSR) oil is crucial to utilization of SSR oil. Aspergillus niger lipase (ANL) has been widely applied for such purpose while its immobilization still has large room for improvement. ANL was immobilized onto six different macroporous acrylic resins, accounting the effect of the different textural properties of resins on stability and their potential for application in enzymatic deacidification. The resin MARE with lower porosity, higher bulk density, and medium hydrophobicity, was chosen as the best carrier for the best thermostability and reusability. ANL-MARE is a promising catalyst than Novozym 40086, which not only exhibited higher deacidification activity and good thermostability, but also was continuously reused for 15 cycles and efficiently catalyzed from high-acid SSR oil into diacylglycerol-enriched oil. Therefore, immobilized ANL was a novel, low-cost and recyclable biocatalyst that could be used as a good alternative to higher-cost commercial lipases in industrial applications.

中文翻译：

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将黑曲霉脂肪酶固定在新型大孔丙烯酸树脂上：稳定且可回收的生物催化，用于高酸酱油渣油的脱酸。

高酸酱油渣油（SSR）的脱酸对于SSR油的利用至关重要。黑曲霉脂肪酶（ANL）已被广泛用于此目的，而其固定化仍然有很大的改进空间。将ANL固定在六种不同的大孔丙烯酸树脂上，说明了树脂不同质地特性对稳定性的影响及其在酶促脱酸中的应用潜力。具有较低孔隙率，较高堆积密度和中等疏水性的树脂MARE被选为具有最佳热稳定性和可重复使用性的最佳载体。ANL-MARE是比Novozym 40086更有前途的催化剂，它不仅具有较高的脱酸活性和良好的热稳定性，而且还连续重复使用了15个循环，并由高酸SSR油高效催化成富含二酰基甘油的油。因此，固定化的ANL是一种新颖，低成本且可回收的生物催化剂，可在工业应用中用作成本较高的商业脂肪酶的良好替代品。