Rice allelopathy is a natural method of weed control that is regarded as an eco-friendly practice in agroecology. The allelopathic potential of rice is regulated by various genes, including those that encode transcription factors. Our study characterized a MYB transcription factor, OsMYB57, to explore its role in the regulation of rice allelopathy. Increasing the expression of OsMYB57 in rice using the transcription activator VP64 resulted in increased inhibitory ratios against barnyardgrass. The gene expression levels of OsPAL, OsC4H, OsOMT, and OsCAD from the phenylpropanoid pathway were also up-regulated, and the content of l-phenylalanine increased. Chromatin immunoprecipitation incorporated with HiSeq demonstrated that OsMYB57 transcriptionally regulated a mitogen-activated protein kinase (OsMAPK11); in addition, OsMAPK11 interacted with OsPAL2;3. The expression of OsPAL2;3was higher in the allelopathic rice PI312777 than in the non-allelopathic rice Lemont, and OsPAL2;3 was negatively regulated by Whirly transcription factors. Moreover, microbes with weed-suppression potential, including Penicillium spp. and Bacillus spp., were assembled in the rhizosphere of the rice accession Kitaake with increased expression of OsMYB57, and were responsible for phenolic acid induction. Our findings suggest that OsMYB57 positively regulates rice allelopathy, providing an option for the improvement of rice allelopathic traits through genetic modification.

中文翻译：

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MYB57转录调节MAPK11与PAL2; 3相互作用并调节水稻化感作用。

水稻化感作用是控制杂草的一种自然方法，在农业生态学中被认为是一种生态友好的做法。水稻的化感潜力由多种基因调节，包括编码转录因子的基因。我们的研究对MYB转录因子OsMYB57进行了表征，以探讨其在水稻化感作用中的调控作用。使用转录激活因子VP64增加水稻中OsMYB57的表达会导致对bar草的抑制率增加。苯丙烷途径的OsPAL，OsC4H，OsOMT和OsCAD的基因表达水平也被上调，而1-苯丙氨酸的含量增加。结合HiSeq的染色质免疫沉淀表明，OsMYB57转录调节有丝分裂原激活的蛋白激酶（OsMAPK11）；此外，OsMAPK11与OsPAL2; 3相互作用。化感水稻PI312777中的OsPAL2; 3表达高于非化感水稻Lemont，而OsPAL2; 3受Whirly转录因子的负调控。此外，具有除草潜力的微生物，包括青霉菌。芽孢杆菌和芽孢杆菌属（Bacillus spp。）被组装在水稻品种北岳（Kataake）的根际中，其OsMYB57的表达增加，并负责酚酸的诱导。我们的发现表明，OsMYB57积极调节水稻化感作用，为通过基因修饰改善水稻化感性状提供了选择。具有抑制杂草作用的微生物，包括青霉菌。芽孢杆菌和芽孢杆菌属（Bacillus spp。）被组装在水稻品种北岳（Kataake）的根际中，其OsMYB57的表达增加，并负责酚酸的诱导。我们的发现表明，OsMYB57积极调节水稻化感作用，为通过基因修饰改善水稻化感性状提供了选择。具有抑制杂草作用的微生物，包括青霉菌。芽孢杆菌和芽孢杆菌属（Bacillus spp。）被组装在水稻品种北岳（Kataake）的根际中，其OsMYB57的表达增加，并负责酚酸的诱导。我们的发现表明，OsMYB57积极调节水稻化感作用，为通过基因修饰改善水稻化感性状提供了选择。