Ticks transmit more pathogens than any other arthropod vector and are of paramount veterinary and zoonotic significance. Domestic dogs are an important reservoir of zoonotic agents, particularly because of their close contact with humans. Previous studies in Korea have examined canine tick-borne pathogens (CTBP) by ELISA and real-time PCR methods. However, phylogenetic information on CTBP in Korea is lacking. This study assessed the prevalence, risk factors, and co-infectivity of CTBP such as piroplasms, rickettsiae, Coxiella burnetii, hepatozoa, hemotropic mycoplasmas, and Borrelia spp., using PCR and phylogenetic analyses. Of the 510 dogs tested, three CTBP, including Anaplasma phagocytophilum 16S rRNA (13; 2.6 %), Hepatozoon canis 18S rRNA (2; 0.4 %), and Mycoplasma haemocanis 16S rRNA (1; 0.2 %) were detected. PCR adapted to amplify A. phagocytophilum msp2 and groEL genes generated amplicons thereof in two out of 13 positive dogs. One shelter dog was co-infected with A. phagocytophilum and M. haemocanis. However, Ehrlichia spp., Rickettsia spp., C. burnetii, Borrelia spp., Babesia spp., and Theileria spp. pathogens were not detected. To the best of our knowledge, this is the first study to undertake a phylogenetic analysis of H. canis and M. haemocanis in dogs reared in Korea. Although previous studies have improved our understanding of evolutionary behaviors and host-pathogen relations of CTBP, additional investigations are required to pin down vectors and reservoirs of CTBP in Korea. A surveillance system for arthropod vectors and CTBP in dogs should be established to monitor pathogen distribution and mitigate pathogen spread proactively, such as with ectoparasite medications in dogs.

壁虱传播的病原体比任何其他节肢动物媒介都要多，并且具有极为重要的兽医和人畜共患病意义。家犬是重要的人畜共患病源，尤其是因为它们与人的密切接触。韩国先前的研究已经通过ELISA和实时PCR方法检查了犬tick传播的病原体（CTBP）。但是，韩国缺乏有关CTBP的系统发育信息。这项研究使用PCR和系统发育分析评估了CTBP的患病率，危险因素和共感染性，例如子质，立克次氏体，伯氏柯立氏菌，肝炎，嗜血性支原体和疏螺旋体。在测试的510只狗中，有3个CTBP，包括嗜食性无浆膜16S rRNA（13; 2.6％），犬肝炎检测到18S rRNA（2; 0.4％）和嗜血支原体16S rRNA（1; 0.2％）。在13只阳性狗中的两只中，适于扩增噬菌嗜性粒细胞msp2和groEL基因的PCR产生了其扩增子。一只避难犬被吞噬嗜血曲霉和血分枝杆菌共同感染。然而，埃立克体属，立克次氏体属，贝氏柯克斯，疏螺旋体属，巴贝虫属和泰勒虫属。未检测到病原体。据我们所知，这是第一次进行的系统发育分析H.犬和在韩国饲养的狗中的血分枝杆菌。尽管以前的研究提高了我们对CTBP进化行为和宿主-病原体关系的理解，但仍需要进行其他调查来确定韩国CTBP的病媒和宿主。应建立犬节肢动物媒介物和CTBP的监测系统，以监测病原体的分布并主动减轻病原体的传播，例如在犬体内使用体外寄生虫药物。