In this study, we have developed a rapid and cost-effective method employing platinum ion (Pt4+)-capped fluorescent carbon quantum dots (CQDs) coupled with loop-mediated isothermal amplification (LAMP) to detect dual MRSA genes. We synthesized nitrogen- and chlorine-co-doped fluorescent CQDs (CQDSPDs) from spermidine trihydrochloride via a simple one-step pyrolysis. The CQDSPDs capped with Pt4+ ions through the cooperative coordination of the amine and chlorine groups on the surface of CQDs facilitated the double-stranded DNA (dsDNA)-induced fluorescence quenching of CQDs, and enabled the construction of the CQDSPDs/Pt4+ probe for the detection of as few as 10 copies of the MRSA gene (mecA and femA). The sensitivity and specificity of the CQDSPDs/Pt4+ probe for MRSA detection in clinical specimens (n = 24) were 94% and 86%, respectively. Our results reveal that the CQDSPDs/Pt4+ probe has great potential for the diagnosis of antibiotic-resistant superbugs with high sensitivity, specificity, and agreement.

中文翻译：

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铂离子介导DNA与碳量子点之间的相互作用：诊断MRSA感染。

在这项研究中，我们已经开发出了一种快速且经济高效的方法，该方法利用铂离子（Pt4 +）封端的荧光碳量子点（CQD）结合环介导的等温扩增（LAMP）来检测双重MRSA基因。我们通过一个简单的一步热解反应，从三氯化亚精胺合成了氮和氯共掺杂的荧光CQD（CQDSPD）。通过CQD表面上的胺和氯基团的协同配位而被Pt4 +离子封端的CQDSPD促进了双链DNA（dsDNA）诱导的CQD荧光猝灭，并使得能够构建CQDSPDs / Pt4 +探针进行检测最多可复制10个MRSA基因（mecA和femA）。CQDSPDs / Pt4 +探针对临床标本（n = 24）中MRSA检测的敏感性和特异性分别为94％和86％。