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Highly sensitive and selective non-enzymatic glucose detection based on indigo carmine/hemin/H2O2 chemiluminescence.
Analyst ( IF 3.6 ) Pub Date : 2019-12-17 , DOI: 10.1039/c9an02100k Tadesse Haile Fereja 1 , Shimeles Addisu Kitte , Muhammad Nadeem Zafar , Mohamed Ibrahim Halawa , Shuang Han , Wei Zhang , Guobao Xu
Analyst ( IF 3.6 ) Pub Date : 2019-12-17 , DOI: 10.1039/c9an02100k Tadesse Haile Fereja 1 , Shimeles Addisu Kitte , Muhammad Nadeem Zafar , Mohamed Ibrahim Halawa , Shuang Han , Wei Zhang , Guobao Xu
Affiliation
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A new chemiluminescence (CL) system, indigo carmine/glucose/hemin/H2O2, has been found and developed for non-enzymatic detection of indigo carmine (IC) and glucose. The CL response increases linearly with IC concentrations from 3.2 μM to 10 mM and glucose concentrations from 0.06 μM to 3.5 mM. The detection limits are 1.45 μM and 15.0 nM for IC and glucose, respectively. This method allows the determination of glucose in blood and urine after simple dilution. The recoveries for the determination of glucose are between 98.5% and 101.0% in blood and between 98.5% and 101.3% in urine. This method shows good sensitivity, selectivity, simplicity, and is low cost, suggesting its promising broad applications.
中文翻译：
基于靛蓝胭脂红/血红素/ H2O2化学发光的高度灵敏且选择性的非酶法葡萄糖检测。
已经发现并开发了一种新的化学发光（CL）系统，靛蓝胭脂红/葡萄糖/血红素/ H2O2，用于非酶检测靛蓝胭脂红（IC）和葡萄糖。CL响应随着IC浓度从3.2μM到10 mM和葡萄糖浓度从0.06μM到3.5 mM线性增加。IC和葡萄糖的检出限分别为1.45μM和15.0 nM。该方法允许简单稀释后测定血液和尿液中的葡萄糖。测定葡萄糖的回收率在血液中为98.5％至101.0％，在尿液中为98.5％至101.3％。该方法显示出良好的灵敏度，选择性，简便性和低成本，表明其有希望的广泛应用。
更新日期：2020-02-13
中文翻译：
基于靛蓝胭脂红/血红素/ H2O2化学发光的高度灵敏且选择性的非酶法葡萄糖检测。
已经发现并开发了一种新的化学发光（CL）系统，靛蓝胭脂红/葡萄糖/血红素/ H2O2，用于非酶检测靛蓝胭脂红（IC）和葡萄糖。CL响应随着IC浓度从3.2μM到10 mM和葡萄糖浓度从0.06μM到3.5 mM线性增加。IC和葡萄糖的检出限分别为1.45μM和15.0 nM。该方法允许简单稀释后测定血液和尿液中的葡萄糖。测定葡萄糖的回收率在血液中为98.5％至101.0％，在尿液中为98.5％至101.3％。该方法显示出良好的灵敏度，选择性，简便性和低成本，表明其有希望的广泛应用。
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