Ara h 1 is recognized as a major peanut allergen. The effects of high hydrostatic pressure (HHP) on the immunoreactivity and structure of Ara h 1 were investigated in this study. The immunoreactivity of Ara h 1 was considerably reduced (P < 0.01) after HHP treatment (≥ 400 MPa) with the increase of pressure holding time. The maximum reduction of 74.32% was achieved at 600 MPa for 1200 s. The particle size and molecular weight of Ara h 1 increased, and the secondary/tertiary structure changed markedly. The decrease of the immunoreactivity of Ara h 1 was mainly due to the changes in conformation (especially the tertiary structure) and the formation of new multimers, resulting in the inactivation of immunoreactive sites. Results indicate that HHP technology has the potential to be applied to the preparation of hypoallergenic peanut products.

中文翻译：

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高静水压处理对花生主要变应原Ara h 1免疫反应性和空间结构的影响

Ara h 1被认为是主要的花生过敏原。本研究研究了高静水压（HHP）对Ara h 1免疫反应性和结构的影响。Ara h 1的免疫反应性大大降低（P HHP处理（≥400 MPa）后，随着保压时间的增加，<0.01。在600 MPa下持续1200 s时，最大降低量达到74.32％。Ara h 1的粒径和分子量增加，二级/三级结构显着变化。Ara h 1免疫反应性的降低主要是由于构象的变化（尤其是三级结构）和新的多聚体的形成，导致免疫反应位点的失活。结果表明，HHP技术有潜力用于制备低变应原性花生产品。