Brightness-gated two-color coincidence detection unravels two distinct mechanisms in bacterial protein translation initiation.,Communications Biology

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Brightness-gated two-color coincidence detection unravels two distinct mechanisms in bacterial protein translation initiation.
Communications Biology ( IF 5.2 ) Pub Date : 2019-12-06 , DOI: 10.1038/s42003-019-0709-7
Henning Höfig _{1,

2} , Olessya Yukhnovets _{1,

2} , Cristina Remes _{2,

3} , Noemie Kempf _{2,

4} , Alexandros Katranidis ₂ , Daryan Kempe _{1,

5} , Jörg Fitter _{1,

2}

Affiliation

Life on the molecular scale is based on a complex interplay of biomolecules under which the ability of binding is crucial. Fluorescence based two-color coincidence detection (TCCD) is commonly used to characterize molecular binding, but suffers from an underestimation of coincident events. Here, we introduce a brightness-gated TCCD which overcomes this limitation and benchmark our approach with two custom-made calibration samples. Applied to a cell-free protein synthesis assay, brightness-gated TCCD unraveled a previously disregarded mode of translation initiation in bacteria.

中文翻译：

亮度门控双色重合检测揭示了细菌蛋白质翻译起始的两种不同机制。

分子尺度上的生命基于生物分子复杂的相互作用，其中结合能力至关重要。基于荧光的双色重合检测 (TCCD) 通常用于表征分子结合，但存在低估重合事件的问题。在这里，我们引入了亮度门控 TCCD，它克服了这一限制，并用两个定制的校准样本对我们的方法进行了基准测试。亮度门控 TCCD 应用于无细胞蛋白质合成测定，揭示了以前被忽视的细菌翻译起始模式。

更新日期：2019-12-06

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