Comparative Proteomics Unravels the Differences in Salt Stress Response of Own-Rooted and 110R-Grafted Thompson Seedless Grapevines.,Journal of Proteome Research

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Comparative Proteomics Unravels the Differences in Salt Stress Response of Own-Rooted and 110R-Grafted Thompson Seedless Grapevines.
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2019-12-20 , DOI: 10.1021/acs.jproteome.9b00420
Sucheta Patil _{1,

2} , Manisha Shinde ₃ , Ramya Prashant ₁ , Narendra Kadoo _{1,

2} , Anuradha Upadhyay ₃ , Vidya Gupta _{1,

2}

Affiliation

Thompson Seedless, a commonly grown table grape variety, is sensitive to salinity when grown on its own roots, and therefore, it is frequently grafted onto salinity-tolerant wild grapevine rootstocks. Rising soil salinity is a growing concern in irrigated agricultural systems. The accumulation of salts near the root zone severely hampers plant growth, leading to a decrease in the productive lifespan of grapevine and causing heavy yield losses to the farmer. In the present study, we investigated the differences in response to salinity between own-rooted Thompson Seedless (TSOR) and 110R-grafted Thompson Seedless (TS110R) grapevines, wherein 110R is reported to be a salt-tolerant rootstock. The grapevines were subjected to salt stress by treating them with a 150 mM NaCl solution. The stress-induced changes in protein abundance were investigated using a label-free shotgun proteomics approach at three time-points viz. 6 h, 48 h, and 7 days of salt treatment. A total of 2793 proteins were identified, of which 246 were differentially abundant at various time-points in TSOR and TS110R vines. The abundance of proteins involved in several biological processes such as photosynthesis, amino acid metabolism, translation, chlorophyll biosynthesis, and generation of precursor metabolites was significantly affected by salt stress in both the vines but at different stages of stress. The results revealed that TSOR vines responded fervently to salt stress, while TS110R vines adopted a preventive approach. The findings of this study add to the knowledge of salinity response in woody and grafted plants and hence open the scope for further studies on salt stress-specific differences induced by grafting.

中文翻译：

比较蛋白质组学揭示了自生根和110R嫁接的汤普森无核葡萄的盐胁迫响应差异。

汤普森无核种（Thompson Seedless）是一种常见的食用葡萄品种，在其自身的根部生长时对盐分敏感，因此，经常将其嫁接到耐盐碱度的野生葡萄根茎上。在农业灌溉系统中，土壤盐碱度的上升越来越引起人们的关注。根部区域附近盐分的积累严重阻碍了植物的生长，导致葡萄的生产寿命缩短，并给农民造成了严重的产量损失。在本研究中，我们调查了自生根的汤普森无核（TSOR）和110R嫁接的汤普森无核（TS110R）葡萄对盐度的响应差异，其中110R被报道是耐盐的砧木。通过用150 mM NaCl溶液处理葡萄，使其经受盐胁迫。使用无标记shot弹枪蛋白质组学方法在三个时间点对应激诱导的蛋白质丰度变化进行了研究。盐处理分别为6小时，48小时和7天。鉴定出总共2793种蛋白质，其中246种在TSOR和TS110R葡萄的不同时间点差异丰富。盐分胁迫在两个葡萄藤中均处于不同的胁迫阶段，这些蛋白质参与了许多生物过程，例如光合作用，氨基酸代谢，翻译，叶绿素生物合成以及前体代谢产物的产生，这些蛋白质的丰度受到显着影响。结果表明，TSOR葡萄藤对盐胁迫反应强烈，而TS110R葡萄藤则采取了预防措施。

更新日期：2019-12-21

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